recombineering-homologous-recombination-constructs-in-drosophila

Recombineering Homologous Recombination Constructs in <em>Drosophila</em>

The continued development of techniques for fast, large-scale manipulation of endogenous gene loci will broaden the use of Drosophila melanogaster as a ...

University of Texas Southwestern Medical Center at Dallas

Nella linea germinale femminile della drosofila, spazialmente e temporalmente specifico traduzione di mRNA governa sia il mantenimento delle cellule staminali e la differenziazione della loro progenie. Tuttavia, i meccanismi che controllano e coordinano i diversi modi di repressione traslazionale nell&#39;ambito di questo lignaggio restano non completamente capiti. Qui di seguito presentiamo i dati mostrando che Mei-P26 associa a Bam, Bgcn e Sxl e nanos mRNA durante lo sviluppo iniziale di cisti, suggerendo che questa proteina aiuta a reprimere la traduzione di nanos mRNA. Insieme a studi recentemente pubblicati, questi dati suggeriscono che Mei-P26 da intermediario sia differenziazione di auto-rinnovamento e germinali GSC attraverso distinte modalit&agrave; di repressione traslazionale a seconda della presenza di Bam.

Mei-p26 collabora con Bam, Bgcn e Sxl per promuovere lo sviluppo iniziale di germinali nell&#39;ovaio di Drosophila.

Dans la lign&eacute;e germinale femelle de drosophile, sp&eacute;cifique dans le temps et l&#39;espace traduction des ARNm r&eacute;git les entretien de cellules souches et la diff&eacute;renciation de leur prog&eacute;niture. Cependant, les m&eacute;canismes qui contr&ocirc;lent et coordonnent les diff&eacute;rents modes de r&eacute;pression traductionnelle dans cette lign&eacute;e restent incompl&egrave;tement compris. Nous pr&eacute;sentons des donn&eacute;es montrant que Mei-P26 associe &agrave; Bam, Bgcn et Sxl et nanos ARNm au d&eacute;but du d&eacute;veloppement kyste, ce qui sugg&egrave;re que cette prot&eacute;ine aide &agrave; r&eacute;primer la traduction de nanos ARNm. Ainsi que des &eacute;tudes publi&eacute;es r&eacute;cemment, ces donn&eacute;es sugg&egrave;rent que Mei-P26 intervient dans les deux diff&eacute;renciation de GSC autorenouvellement et dans les cellules germinales dans des modes distincts de r&eacute;pression traductionnelle selon la pr&eacute;sence de Bam.

Mei-p26 coop&egrave;re avec Bam, Bgcn et Sxl &agrave; la promotion du d&eacute;veloppement de la lign&eacute;e germinale dans l&#39;ovaire de la drosophile.

In the Drosophila female germline, spatially and temporally specific translation of mRNAs governs both stem cell maintenance and the differentiation of their progeny. However, the mechanisms that control and coordinate different modes of translational repression within this lineage remain incompletely understood. Here we present data showing that Mei-P26 associates with Bam, Bgcn and Sxl and nanos mRNA during early cyst development, suggesting that this protein helps to repress the translation of nanos mRNA. Together with recently published studies, these data suggest that Mei-P26 mediates both GSC self-renewal and germline differentiation through distinct modes of translational repression depending on the presence of Bam.

Arnaldo Carreira-Rosario

Department of Molecular Biology

University of Texas Southwestern Medical Center at Dallas

Recombineering Homologous Recombination Constructs in Drosophila

Arnaldo Carreira-Rosario

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Molecular Biology

University of Texas Southwestern Medical Center at Dallas

Recombineering Homologous Recombination Constructs in Drosophila

Department of Molecular Biology