Worcester Polytechnic Institute- WPI

2 ARTICLES PUBLISHED IN JoVE

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Biology

Heterokaryon Technique for Analysis of Cell Type-specific Localization
Roseann Gammal *1, Krista Baker *1, Destin Heilman 1
1Department of Chemistry and Biochemistry, Worcester Polytechnic Institute- WPI

A flexible and efficient method for the characterization of cell type-specific protein localization and nucleocytoplasmic shuttling is described. This heterokaryon approach uses fluorescently-labeled fusion proteins to image protein localizations after cell fusion. The protocol is amenable to steady-state localizations or more dynamic determinations based on live cell imaging.

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Biology

Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens
Yen-Chun Liu 1, Luis Vidali 1
1Department of Biology and Biotechnology, Worcester Polytechnic Institute- WPI

A simple and efficient method to transform Physcomitrella pantens protoplasts is described. This method is adapted from protocols for Physocmitrella protonemal protoplast and Arabidopsis mesophyll protoplast transformation1.

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