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The Salk Institute

1 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Chemical Triphosphorylation of Oligonucleotides
Grant A. L. Bare 1, David P. Horning 1
1The Salk Institute

Oligonucleotide 5′-triphosphates are ubiquitous components in essential biological pathways and have seen increasing use in biotechnology applications. Here, we describe techniques for the routine synthesis and purification of oligonucleotide 5′-triphosphates, starting from oligonucleotides prepared by standard automated synthesis techniques.

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