biology

Biology

cancer research

Cancer Research

biological techniques

Biological Techniques

immunology

Immunology

neuroscience

Neuroscience

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jove encyclopedia of experiments

JoVE Encyclopedia of Experiments

Online video encyclopedias of advanced research experiments for scientists in academia and biotech

Research

genome editing techniques

This collection features techniques used for genome editing in cultured bacterial, animal, and plant cells and in vivo animal models. These methods highlight the applications of CRISPR technology, Zinc finger nucleases, TALENs, transposition and recombination events, shRNA, siRNA, and vector-based transformations to achieve gene knock-in, gene knock-out, and gene regulations.

Genome Editing Techniques

gene transfer techniques

This collection features gene transfer methods, including viral vector-mediated transduction and non-viral vector-mediated physical and chemical transfection techniques to deliver genetic material in cultured cells and in vivo animal models at their embryonic and adult stages. Highlights include transfection utilizing hydrodynamics, microinjection, biolistics, nucleofection, and magnetofection techniques, and transduction via retroviral, adenoviral, nanoblade, and lentiviral systems.

Gene Transfer Techniques

staining techniques

Staining Techniques

This collection features methods for staining biomolecules such as lipids, carbohydrates, and proteins; and different cell types ranging from individual plant or animal cells to various tissue sections. These methods help visualize and characterize a wide variety of biological samples by improving their contrast.

chromatography techniques

This collection explains the mechanisms behind separating and purifying various analytes such as proteins, lipids, carbohydrates, antibody-drug conjugates, and small molecules from complex mixtures using column, capillary, or plate-based systems. The highlights include chromatographic separation methods using ion exchange, standard and reverse-phase, affinity, size-exclusion, gas-based, and thin-layer dependent techniques.

Chromatography Techniques

electrophoresis techniques

This collection highlights the mechanisms behind various electrophoresis techniques to separate biomolecules such as DNA, RNA, peptides, individual proteins, protein complexes, and amyloids. These methods utilize variants of agarose gel- or polyacrylamide gel-based matrices to separate biomolecules under the influence of an electric field.

Electrophoresis Techniques

assay techniques

This collection features various bioanalytical techniques to assess the activity, potency, or localization of test substances in living organisms performed at the macromolecular, sub-cellular, cellular, or organism level. Assay outcomes are detected directly or indirectly by visualizing the responses via colorimetric, enzymatic, or electrophysiological measurement methods.

Assay Techniques

pcr techniques

The focus of this collection extends to a range of applications and methods that utilize Polymerase Chain Reaction (PCR) to identify and examine genetic markers, mutations, and sequences. These techniques encompass mutation detection, annealing temperature optimization, genetic marker quantification, infection diagnosis, enumeration of infective particles, nucleic acid amplification, analysis of epigenetic modifications, and detection of transcript variants.

PCR Techniques

blot techniques

Blot techniques encompass a variety of meticulous methods employed for the quantification and detection of biomolecules, spanning DNA, RNA, and proteins, contributing to a comprehensive understanding of biological systems. This collection features diverse applications, including the precise quantification and detection of biomolecules, assessing genetic material, examining epigenetic modifications, and measuring protein expression levels.

Blot Techniques

biomolecular interaction detection techniques

This collection features various methods and techniques used to study protein-protein interactions. These approaches encompass scientific tools and technologies, such as biosensors, imaging techniques, assays, and spectroscopy, that provide insights into the intricate world of protein interactions and their roles in biological systems, enabling a deeper understanding of the events that transpire at the molecular level. These interactions are fundamental in numerous cellular activities, including signal transduction, enzymatic reactions, gene regulation, and structural organization.

Biomolecular Interaction Detection Techniques

spectroscopy techniques

This collection highlights various spectroscopy techniques that are relevant in molecular biology. Based on the principle that molecules absorb or emit light at specific wavelengths or frequencies, spectroscopy allows for the measurement of light absorption, emission, or scattering. These techniques provide valuable insights into the composition, structure, interactions, and dynamics of molecules involved in biological processes.

Spectroscopy Techniques

microscopy techniques

The collection comprises an array of advanced experimental techniques in the field of microscopy and imaging, aimed at investigating various biological and cellular processes. These techniques enable researchers to visualize and study dynamic cellular events, molecular interactions, and structural features with high resolution and specificity..

Microscopy Techniques

gene knock-in

Gene knock-in

gene knock-out

Gene knock-out

gene regulation

Gene regulation

all-in-one crispr genome editing: a method for homology directed repair-based gene knock-in in cultured cells using crispr-cas9 system

all-one-crispr-genome-editing-method-for-homology-directed-repair

All-in-One CRISPR Genome Editing: A Method for Homology Directed Repair-Based Gene Knock-In in Cultured Cells Using CRISPR-Cas9 System

Source: Zhang, L. et al, Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines. J. Vis. Exp. (2021).
In this video, we demonstrate ...

crispr-mediated base editing tools: a genome editing technique to induce targeted base substitution

crispr-mediated-base-editing-tools-genome-editing-technique-to-induce

CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution

Source: See, J. E., et al. Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors. J. Vis. Exp. (2021).
This video explains the ...

zinc finger nuclease-based genome editing: a technique for modifying genome in human pluripotent stem cells by double-stranded homology dependant repair mechanism

zinc-finger-nuclease-based-genome-editing-technique-for-modifying

Zinc Finger Nuclease-Based Genome Editing: A Technique for Modifying Genome in Human Pluripotent Stem Cells by Double-Stranded Homology Dependant Repair Mechanism

Source: Blair, J. D., et al. Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation. J. Vis. Exp. (2016).
This ...

talen-mediated targeted gene integration: using engineered sequence-specific nucleases for the precise insertion of fluorescent protein gene into hipscs

talen-mediated-targeted-gene-integration-using-engineered-sequence

TALEN-Mediated Targeted Gene Integration: Using Engineered Sequence-Specific Nucleases for the Precise Insertion of Fluorescent Protein Gene into hiPSCs

Source: Cerbini, T. et al. Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the ...

phic31-integrase-mediated site-directed transgene integration: a microinjection technique for site-specific transgene integration into an anopheles vector

phic31-integrase-mediated-site-directed-transgene-integration

phiC31-Integrase-Mediated Site-Directed Transgene Integration: A Microinjection Technique for Site-Specific Transgene Integration into an Anopheles Vector

Source: Adolfi, A., Lynd, A., Lycett, G. J., James, A. A. Site-Directed &#966;C31-Mediated Integration and Cassette Exchange in Anopheles Vectors of ...

binary bacterial artificial chromosome vector based gene transformation in arabidopsis plants: a technique to generate transgenic plants with single-copy insertion

binary-bacterial-artificial-chromosome-vector-based-gene

Binary Bacterial Artificial Chromosome Vector Based Gene Transformation in Arabidopsis Plants: A Technique to Generate Transgenic Plants With Single-Copy Insertion

Source: Tark-Dame, M., et al. Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector. J. Vis. Exp. (2018).
In this video, ...

tol2 transposon-mediated zebrafish transgenesis: a procedure to generate transgenic zebrafish following the coinjection of tol2 system into fertilized zebrafish embryos

tol2-transposon-mediated-zebrafish-transgenesis-procedure-to-generate

Tol2 Transposon-Mediated Zebrafish Transgenesis: A Procedure to Generate Transgenic Zebrafish Following the Coinjection of Tol2 System Into Fertilized Zebrafish Embryos

Source: Silic, M. R., et al.  Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors. J. Vis. Exp. (2018)
In this video, we ...

piggybac transposon-mediated gene editing in human ipscs: a procedure to integrate gene of interest in human ipscs using piggybac transposon system

piggybac-transposon-mediated-gene-editing-human-ipscs-procedure-to

PiggyBac Transposon-Mediated Gene Editing in Human iPSCs: A Procedure to Integrate Gene of Interest in Human iPSCs Using PiggyBac Transposon System

Source: Garone, M. G. et al. Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac ...

crispr concatemer-mediated multiple gene knockout: a technique to simultaneously knockout multiple genes by non-homologous end-joining pathway in mouse intestinal cells

crispr-concatemer-mediated-multiple-gene-knockout-technique-to

CRISPR Concatemer-Mediated Multiple Gene Knockout: A Technique to Simultaneously Knockout Multiple Genes by Non-Homologous End-Joining Pathway in Mouse Intestinal Cells

Source: Merenda, A. et al., A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer. J. Vis. Exp. (2017).
This ...

crispr interference-based gene silencing: a technique for targeted repression of gene function in pathogenic leptospira

crispr-interference-based-gene-silencing-technique-for-targeted

CRISPR Interference-Based Gene Silencing: A Technique for Targeted Repression of Gene Function in Pathogenic Leptospira

Source: Fernandes, L. G. V., et al. Application of CRISPR Interference (CRISPRi) for Gene Silencing in Pathogenic Species of Leptospira. J. Vis. Exp. ...

creer-loxp system-based target gene inactivation: a tamoxifen-inducible cre recombinase system for target gene knockout in a mouse model

creer-loxp-system-based-target-gene-inactivation-tamoxifen-inducible

CreER-LoxP System-Based Target Gene Inactivation: A Tamoxifen-Inducible Cre Recombinase System for Target Gene Knockout in a Mouse Model

Source: Hubner, E. K., et al. Constitutive and Inducible Systems for Genetic&#160;In Vivo&#160;Modification of Mouse Hepatocytes Using Hydrodynamic Tail ...

short hairpin rna-mediated gene knockdown in ihspcs in vitro: a lentivirus-based shrna expression system delivery into ihspcs for knockdown of specific gene expression

short-hairpin-rna-mediated-gene-knockdown-ihspcs-vitro-lentivirus

Short Hairpin RNA-Mediated Gene Knockdown in iHSPCs In Vitro: A Lentivirus-Based shRNA Expression System Delivery into iHSPCs for Knockdown of Specific Gene Expression

Source: Hsiao, Y. L., et al.  Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell ...

inducible tet-on regulatable system-based gene expression in vivo: a tetracycline-induced gene expression system for target gene transcription activation in mouse model

inducible-tet-on-regulatable-system-based-gene-expression-vivo

Inducible Tet-On Regulatable System-Based Gene Expression In Vivo: A Tetracycline-Induced Gene Expression System for Target Gene Transcription Activation in Mouse Model

Source: Hubner, E. K. et al.&#160; Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein ...

sirna reverse transfection-based gene silencing in vitro: a procedure to deliver small interfering rna in cultured cells to inactivate target gene expression

sirna-reverse-transfection-based-gene-silencing-vitro-procedure-to

siRNA Reverse Transfection-Based Gene Silencing In Vitro: A Procedure to Deliver Small Interfering RNA in Cultured Cells to Inactivate Target Gene Expression

Source: Jager, J. et al.&#160; An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function. J. Vis. Exp. ...

inducible tet-off regulatable system: an in vitro method to modulate gene expression in cultured cells using tetracycline-off system

inducible-tet-off-regulatable-system-an-vitro-method-to-modulate-gene

Inducible Tet-Off Regulatable System: An In Vitro Method to Modulate Gene Expression in Cultured Cells Using Tetracycline-Off System

Source: Migneault, F. et al, Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in ...

morpholino mediated gene editing: a gene knockdown technique using translational blocking morpholino oligonucleotides in single-celled cavefish eggs

morpholino-mediated-gene-editing-gene-knockdown-technique-using

Morpholino Mediated Gene Editing: A Gene Knockdown Technique Using Translational Blocking Morpholino Oligonucleotides in Single-Celled Cavefish Eggs

Source: Stahl, B. A. et al., Manipulation of Gene Function in Mexican Cavefish. J. Vis. Exp. (2019).
This video describes a gene knockdown technique using ...

Encyclopedia Of Experiments - biological techniques