Experimental Glaucoma Induction via Iridocorneal Angle Obstruction In a Mouse Model

Start with an anesthetized rat with a sterilized eye, and place a magnetic ring covering the eyeball.

Pierce the eye tangentially with a syringe containing magnetic beads.

Inject the beads into the anterior chamber of the eye, holding the syringe in position.

The magnet pulls the beads to the iridocorneal angle and begins to block the outflow of aqueous humor fluid from the eye through the trabecular meshwork.

Angle the needle slightly when removing the syringe to avoid a sudden increase in eye pressure.

Remove the magnet and reposition it to direct beads into areas with incomplete coverage.

Then, place the magnet back around the eyeball.

As the beads settle, they completely block aqueous humor outflow, causing fluid buildup in the anterior chamber and increased eye pressure.

The elevated pressure induces mechanical stress on the retina, promoting optic nerve degeneration and developing a glaucoma-like condition.