Bioluminescence Imaging of Neuroinflammation in a Transgenic Mouse

Begin with an anesthetized transgenic mouse containing pathogenic protein aggregates, which cause neuroinflammation and astrocyte activation, leading to increased luciferase expression.

Remove the fur from its head to prevent light scattering during imaging.

Color the ears with a non-irritating marker to block nonspecific bioluminescence.

Take an appropriate volume of luciferin based on the mouse's body weight and inject it intraperitoneally.

Luciferin enters the bloodstream, reaches the brain, and is taken up by activated astrocytes.

Within the astrocyte, the luciferase oxidizes luciferin, emitting a bioluminescent signal.

Set the imaging parameters and precisely position the mouse on the bioluminescence imaging chamber's heating plate. 

Ensure proper anesthesia flow and capture the bioluminescent signal for the desired duration.

Then, select the region of interest and quantify the targeted bioluminescent signal. 

The intensity of this signal corresponds to luciferase activity, reflecting astrocytic activation and providing a direct measure of neuroinflammation.