Name: Enzymes: Lock-and-key &amp; Induced-fit Models; Enzyme Inhibitors
Uploaded: 2020-09-24T15:06:50
Description: Learn by watching this video about Enzymes: Lock-and-key & Induced-fit Models; Enzyme Inhibitors at JoVE.com

Enzymes are biological catalysts that accelerate the rate of biochemical reactions. Most enzymes are proteins, made up of amino acids; while some are RNA molecules known as ribozymes.&#160;
Enzymes act by lowering the activation energy of a reaction, thereby increasing the reaction rate. They can catalyze both synthesis and breakdown of chemical bonds, but do not affect the direction or equilibrium of the reaction.&#160;
Each enzyme binds to a specific reactant, called the substrate, and catalyzes a particular reaction. The substrate binds to a distinct catalytic region of the enzyme called the active site through interactions such as intermolecular forces and transient covalent bonds, resulting in an enzyme-substrate complex.
This binding is highly specific due to the conformational complementarity required between the enzyme and its substrate. Thus, a particular enzyme can only catalyze specific reactions based on its conformation.&#160;&#160;
A simple way of understanding complex formation is the lock-and-key model, which hypothesizes that the substrate fits into the enzyme&#39;s active site, analogous to a &#39;key&#39; fitting into its corresponding &#39;lock&#39;.&#160;
However, another model, the induced-fit model, takes into account the dynamic nature of the complex. This model states that when a substrate binds, it induces small conformational changes, resulting in a tighter fit that favors the reaction.&#160;
The activation energy for a reaction can be lowered by various methods.&#160;
Common mechanisms include inducing conformational changes in the substrate that allow a bond to be broken more easily or bringing the reactive groups of two substrates in close proximity, thereby promoting bond formation.\Enzyme activity can be temporarily or permanently suppressed by natural or synthetic molecules called inhibitors.
For example, a competitive inhibitor competes with the substrate to bind to the enzyme&#39;s active site, thereby preventing substrate binding.
On the other hand, a noncompetitive inhibitor binds to another location on the enzyme, which causes a conformational change at the active site, reducing the enzyme&#8217;s catalytic activity.

enzymes

Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD (glucose-6-phosphate dehydrogenase) adversely affects the metabolic pathway supplying NADPH to cells.
A disruption in this metabolic pathway can reduce glutathione in red blood cells causing damage to other enzymes and proteins like hemoglobin. The excessive metabolization of hemoglobin builds up the bilirubin level, which leads to jaundice, a condition that can become severe. Hence, people who suffer from G6PD deficiency must avoid certain foods and medicines containing chemicals that could trigger damage to their glutathione-deficient red blood cells.
Enzyme Function and Structure
Enzymes are grouped into different classes based on the specific function they perform. For example, oxidoreductases are involved in redox reactions, while transferases catalyze the transfer of functional groups. Bond formation with ATP hydrolysis requires ligases, whereas hydrolysis reactions and double bonds formation are catalyzed by hydrolases and lyases, respectively. Isomerase enzymes usually catalyze isomerization reactions.
Enzymes generally possess active sites. These are specific regions on the molecule with a conformation that favors the enzyme to bind to a specific substrate (a reactant molecule) to form an enzyme-substrate complex or the reaction intermediate.
Two models&#8212;the lock-and-key model and the induced-fit model&#8212;attempt to explain the working of an active site (Figure 1). The most simplistic lock-and-key hypothesis suggests that the active site and the molecular shape of the substrate are complementary&#8212;fitting together like a key in a lock (Figure 1a). On the other hand, the induced-fit hypothesis suggests that the enzyme molecule is flexible and changes shape to accommodate a bond with the substrate (Figure 1b).
However, both the lock-and-key model and the induced-fit model account for the fact that enzymes can only bind with specific substrates and catalyzes only a particular reaction.
<img alt="Eq1" src="/files/ftp_upload/11384/11384_Image_1.png" style="text-align: center; height: 300px;" />
Figure 1 (a) According to the lock-and-key model, the shape of an enzyme&#8217;s active site is a perfect fit for the substrate. (b) According to the induced fit model, the active site is somewhat flexible, and can change shape in order to bond with the substrate.
Enzyme Inhibitors
The activity of enzymes can also be interrupted by the process of enzyme inhibition. There are several common types of enzyme inhibition.
During competitive inhibition, a molecule (natural or synthetic) other than the substrate directly binds to the enzyme&#39;s active site. The structural and chemical similarity of the inhibitor to the substrate facilitates its binding to the active site. Such competitive inhibitors, thus, compete with substrates, preventing them from binding to the enzyme. Most often, increasing substrate concentration can suppress the effects of competitive inhibition.
In non-competitive inhibition, a molecule (natural or synthetic) binds to an allosteric (other) region of the enzyme, different from its active site. The inhibitor-binding causes a conformational change to the enzyme&#39;s active site, resulting in a decrease in the enzyme&#39;s ability to catalyze the reaction. Unlike competitive inhibition, an increase in substrate concentration does not mitigate the inhibitory effects of non-competitive inhibition.
Part of this text is adapted from <a href="https://openstax.org/books/chemistry-2e/pages/12-7-catalysis">Openstax, Chemistry 2e, Section 12.7: Catalysis.</a>

Learn by watching this video about Enzymes: Lock-and-key & Induced-fit Models; Enzyme Inhibitors at JoVE.com

Enzymes: Lock-and-key &amp; Induced-fit Models; Enzyme Inhibitors

Enzymes

Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the ...