Hydrolysis of esters under acidic conditions proceeds through a nucleophilic acyl substitution. In the presence of excess water, the reaction proceeds in a reversible manner, forming carboxylic acids and alcohols.

During hydrolysis, the ester is first activated towards nucleophilic attack through the protonation of the carboxyl oxygen atom by the acid catalyst. The protonation makes the ester carbonyl carbon more electrophilic. In the next step, water acts as a nucleophile and adds to the carbonyl carbon, followed by the loss of a proton to give the tetrahedral addition intermediate. Further, the protonation of the alkoxy group converts it into a better leaving group. Subsequently, the carbonyl group is reconstructed with the expulsion of alcohol as a leaving group. Finally,deprotonation yields a carboxylic acidas the final product and regenerates the acid catalyst.

An acid catalyst protonates the carbonyl oxygen, thereby increasing the formation rate of the tetrahedral intermediate. Moreover, the catalyst decreases the basicity of the leaving group. Notably, the reaction is exactly the reverse of a Fischer esterification reaction.