The vaginal canal is a tubular structure averaging about 10 cm in length that acts as the entryway to the female reproductive system and the passageway for menstrual flow and childbirth. The interior walls of the vagina exhibit concentric folds called rugae and are topped by an area known as the fornix, which connects with the protruding cervical portion of the uterus. This canal is comprised of an external fibrous layer, a muscular middle layer, and an inner lining with mucosal rugae, which enable the vagina to stretch during sexual intercourse and the birthing process. The vaginal opening may be partially encircled by the hymen, a thin membrane that can be torn due to various activities, including exercise, sexual activity, and giving birth. The Bartholin's glands, located on either side near the posterior part of the vaginal introitus, secrete lubricating mucus primarily during sexual arousal.

The vaginal environment is typically inhabited by a community of microorganisms that guard against harmful pathogens. In a healthy vagina, the predominant microbes belong to the Lactobacillus genus. Glycogen, stored in the vaginal mucosa, serves as a primary energy source for these beneficial bacteria. Lactobacillus metabolizes glycogen into lactic acid, helping to maintain an acidic pH of less than 4.5. This acidity is detrimental to most pathogens, thereby keeping the environment safe. Mucus, together with lactic acid, supports the vagina's natural self-cleaning process by maintaining a balanced microbial environment. However, the practice of douching can disrupt this balance, increasing the risk of infections and irritation.