eoe sub articles

EoE Sub Articles

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Working Memory Water Maze
<ol>
	<li>Equipment
	<ol>
		<li>Use a tank filled with water to a height of 28 cm and maintained at 26 &#177; 1 &#176;C.</li>
		<li>Use a clear acrylic glass platform that is 10 cm in diameter on a stand 26 cm in height. 
		NOTE: The top surface of the platform shou...

the water maze with platform relocation: a method to assess working memory

Source: Hausser, N., et al. <a href="https://www.jove.com/video/56044/detecting-behavioral-deficits-in-rats-after-traumatic-brain-injury">Detecting Behavioral Deficits in Rats After Traumatic Brain Injury.</a>&#160;J. Vis. Exp (2018).
In this video, we describe the Morris water maze test with platform relocation, a navigational task used to study working memory in rodents.

<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/20049/20049_Figure1.jpg" /> 
Figure 1. Diagram of the water maze.&#160;This diagram demonstrates the possible platform locations (1, 2, 3, 4) and starting points (N, S, E, W) for the working memory Morris water maze. Rats are allowed two trials from each starting location/platform pairing. There is a 15 s inter-trial interval and 4 min rest in a warming chamber between pairs of trials for a total of four pair...

The Water Maze with Platform Relocation: A Method to Assess Working Memory

Source: Hausser, N., et al. Detecting Behavioral Deficits in Rats After Traumatic Brain Injury.&#160;J. Vis. Exp (2018).
In this video, we describe the ...

Ensure visual cues are placed around the water maze tank to help subjects learn the location of the submerged hidden platform. Place the subject in the tank away from the platform facing the wall. The animal will swim to locate the stage and escape.
End the trial after the animal finds the platform or a predetermined time has elapsed. Let the subject stay on top of the platform before repeating the trial a second time. If it fails to locate the stage, guide the animal by hand and allow it to remain on the platform for the duration of the trial.
Then, to assess the animals working memory, repeat the trial a second time. Subjects will have to remember how to locate the platform and use the visual cues as reference. Record the time and path length for repeated trials to use as measures of working memory. After the animal has rested, continue by moving the platform to a new location and perform trials 1 and 2.

The Water Maze with Platform Relocation: A Method to Assess Working Memory

Overview