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In Vivo Murine Liver Perfusion: A Method to Infuse the Rodent Liver with Solution via the Portal Vein
Overview
This video describes a procedure for mouse liver perfusion through the portal vein using collagenase solution prior to isolating liver cells.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Preparation
- Preparation of solutions and culture media
- Prepare all culture media and buffer according to the Table of Materials.
- Preparation of instruments
- Set up a water bath (>10 L) at 42 °C, a peristaltic pump with variable speed, and clam.......
Representative Results
Figure 1: Schematic representation of the perfusion suite. The flasks are held in place by clamps (not shown); the tubing containing fluid is swapped from the 1L flask to the 125 mL flask during the procedure is represented by the red dotted line. Note, that the oxygen feed does not bubble directly into the solutions in the flasks. The corks should also be notched to allow a close.......
This article has been published
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Source: Cabral, F., et al. Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion. J. Vis. Exp. (2018).
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