eoe sub articles

EoE Sub Articles

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Preparation
<ol>
	<li>Preparation of solutions and culture media
	<ol>
		<li>Prepare all culture media and buffer according to the&#160;Table of Materials.</li>
	</ol>
	</li>
	<li>Preparation of instruments
	<ol>
		<li>Set up a water bath (&#62;10 L) at 42 &#176;C, a peristaltic pump with variable speed, and clam...

in vivo murine liver perfusion: a method to infuse the rodent liver with solution via the portal vein

Source: Cabral, F., et al. <a href="https://www.jove.com/video/56993/" target="_blank">Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion</a>. J. Vis. Exp. (2018).
This video describes a procedure for mouse liver perfusion through the portal vein using collagenase solution prior to isolating liver cells.

<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/20084/20084_Figure1.jpg" /> 
Figure 1: Schematic representation of the perfusion suite. The flasks are held in place by clamps (not shown); the tubing containing fluid is swapped from the 1L flask to the 125 mL flask during the procedure is represented by the red dotted line. Note, that the oxygen feed does not bubble directly into the solutions in the flasks. The corks should also be notched to allow a close...

In Vivo Murine Liver Perfusion: A Method to Infuse the Rodent Liver with Solution via the Portal Vein

Source: Cabral, F., et al. Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion. J. Vis. Exp. (2018).
This video ...

After anesthetizing and preparing the rodent, locate the portal vein and slide a thread under it. Tie a loose knot around the portal vein without pulling it taut. This will stabilize an IV catheter later during the protocol.
Holding the needle of an IV catheter bevel-up and parallel to the portal vein, insert it into the lumen - the space inside of the vein. Retract the needle and push the catheter through the vein all the way to the venous branched area within the liver.
After stabilizing the catheter, increase the peristaltic pump rate. Immediately, the liver should blanch as blood is replaced by perfusion liquid. Cut a major blood vessel to drain even more blood and perfusion liquid from the liver.
Squeeze the severed vessel a few times to inflate the liver and ensure it is completely devoid of blood. Switch the pump so that it perfuses buffer into the liver. Finally, repeat liver inflation briefly, taking care not to burst the liver from its connective tissue.

In Vivo Murine Liver Perfusion: A Method to Infuse the Rodent Liver with Solution via the Portal Vein

Overview