eoe sub articles

EoE Sub Articles

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Echocardiography
<ol>
	<li>Determine the body weight of the mouse using a standard laboratory balance while holding it lightly by the tail to ensure proper positioning.</li>
	<li>Anesthetize the animal by the intraperitoneal (i.p.) injection of 50 mg/kg pentobarbital. 
	NOTE: Any other kind of anesthesia can...

mouse echocardiography: a non-invasive technique to assess cardiac morphology

Source: Baudouy, D., et al. <a href="https://www.jove.com/video/55843/" target="_blank">Echocardiographic and Histological Examination of Cardiac Morphology in the Mouse</a>. J. Vis. Exp. (2017).
This video describes a protocol to perform echocardiography on a laboratory mouse.

<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/20096/20096_Figure1.jpg" /> 
Figure 1: Standard-resolution echocardiography (&#62;10 MHz) to measure LV systolic and diastolic dimensions and cardiac function in mice with dilated cardiac hypertrophy after myocardial infarction versus control animals.&#160;Representative echocardiographic recordings from normal, healthy mice (A) and animals with LV dilation and reduced LV function after myoca...

Mouse Echocardiography: A Non-invasive Technique to Assess Cardiac Morphology

Source: Baudouy, D., et al. Echocardiographic and Histological Examination of Cardiac Morphology in the Mouse. J. Vis. Exp. (2017).
This video describes a ...

First, apply echocardiography gel to the ultrasound transducer and to the shaved chest of an anesthetized mouse secured to a warming plate. Place the transducer to the left of the sternum, facing the right side of the neck, to obtain a view through the heart called the parasternal long-axis view.
Turn the transducer 90 degrees clockwise for the parasternal short-axis view. Using this view, record M-mode images to capture movement of the heart over time as it beats. To measure the dimensions of the left ventricle, identify four landmark tissue-fluid interfaces on the recorded images.
These are the interface between the right ventricular cavity and the anterior wall of the left ventricle; between this wall and the left ventricular cavity; between this cavity and the posterior wall of the left ventricle; and between this wall and the pericardium.
Then, calculate the distances between these four interfaces. Repeat the same measurements when the heart is in diastole, or is relaxed, and when it is in systole, or is contracted.

Mouse Echocardiography: A Non-invasive Technique to Assess Cardiac Morphology

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