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Intracranial Orthotopic Model: A Procedure to Implant Cancer Cells in Mouse Brain
Overview
This video demonstrates implantation of tumor cells in the mouse brain by performing intracranial injection.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Injecting Cancer Cells into the Brain
- Place an anesthetized mouse with an exposed calvarial window on the stereotatic frame.
- Attach an automatic injector unit to the stereotactic apparatus (Figure 1).
- Pull up >2 µL of cells thoroughly resuspended in 1x DPBS in a clean Hamilt.......
Representative Results
Figure 1: Stereotactic and anesthesia systems. Stereotactic stand with automated syringe pump and mouse elevating box. The box is an inverted pipette tip box containing hand warmers used to elevate the mouse to the appropriate height and maintain body temperature. Inset shows the orientation of the Hamilton syringe in the automated injection apparatus.
This article has been published
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Source: Geisler, J. A. et al. Modeling Brain Metastases Through Intracranial Injection and Magnetic Resonance Imaging. J. Vis. Exp. (2020)
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