eoe sub articles

EoE Sub Articles

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Solution and Perfusion Apparatus Preparation
<ol>
	<li>Assemble a constant flow Langendorff apparatus (commercially available or self-made).&#160;Figure 1&#160;provides a simple schematic.</li>
	<li>Prepare the solutions according to the reagents given in&#160;Table 1&#160;and&#160;Table ...

langendorff retrograde heart perfusion: an ex vivo technique to perfuse isolated mouse heart

Source: Wu, K. et al. <a href="https://www.jove.com/video/62514" target="_blank">Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice</a>. J. Vis. Exp. (2021)
In this video, we perform Langendorff retrograde perfusion of an isolated mouse heart. During the procedure, the cardiac activity of the heart is maintained by perfusing it retrogradely with a perfusion solution via the coronary arteries using a cannula inserted into the ascending aorta. The model is used to study the cardiac pathophysiology of the heart.

<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/20749/20749_Figure1.jpg" /> 
Figure 1. Schematic diagram of an assembled modified Langendorff system.&#160;This system is economical and portable for the users. It has two parts of plastic tubing-one for the water (inner diameter, 8 mm) and one for the perfusate (inner diameter, 1.5 mm)-of which the distal end is connected to a PE medical three-way stopcock with a Luer lock. A glass bottle containing water an...

Langendorff Retrograde Heart Perfusion: An Ex Vivo Technique to Perfuse Isolated Mouse Heart

Source: Wu, K. et al. Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice. J. Vis. Exp. ...

To begin, place an anesthetized mouse in the supine position on a dissection board. Make an incision to access the thoracic cavity and expose the heart. Locate the aorta and its branches - the left common carotid artery and brachiocephalic artery. Transect these arteries to isolate the heart.
Immediately, immerse the excised heart into a Petri dish containing a nutrient-rich oxygenated buffer solution. Concomitantly, insert a cannula into the heart such that its tip reaches the ascending aorta. Use a suture to ligate the cannula. Connect the cannula to a syringe containing oxygenated perfusion solution.
Inject the perfusion solution into the heart. This step allows the blood to leave the coronary arteries and effuse from the pulmonary veins. Next, connect the cannulated heart to the Langendorff apparatus. Use peristaltic pump to perform perfusion at a constant flow rate.
Under these conditions, the buffer perfuses into the heart. This creates cannulation pressure that closes the aortic valves, causing the buffer to move through the coronary arteries. Eventually, the buffer perfuses the heart muscles retrogradely and later effuses through the pulmonary veins. A successfully perfused heart is now ready for further downstream applications.

Langendorff Retrograde Heart Perfusion: An Ex Vivo Technique to Perfuse Isolated Mouse Heart

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