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Colonoscopic Guided Pinch Biopsy in a Mouse Model: A Technique to Visualize and Extract Tissue Samples from Mouse Colon
Overview
This video demonstrates the protocol for performing colonoscope-guided pinch biopsy in the mouse. This technique helps visually analyze the colon lumen and collect tissue samples for detailed studies.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Colonoscopy and wound induction
- Pre-assemble the components of the endoscope by first inserting the 1.9 mm rigid bore endoscope into the sheath (Figure 1A-B). Attach the air pump (to provide colonic insufflation) using the tubing provided, to the gas valve on the left side of the sheath next to the working channel (
Representative Results
Figure 1: Items needed to carry out biopsies. (A) Image of lens (a), sheath (b) and biopsy forceps (c). (B) Insertion of the lens into the sheath. (C) Insertion of forceps through the working channel of the sheath (solid arrow). Dashed arrow indicates correct location for attachment of air pump.
This article has been published
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Source: Montrose, D. C., et al. Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes. J. Vis. Exp. (2021)
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