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Overview

This video describes orthotopic kidney auto-transplantation in a porcine model. This surgical procedure involves the transplantation of an animal's kidney graft at its original anatomical location. The model is used to investigate new treatments for end-stage kidney diseases in humans.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Basic techniques and common procedures

  1. Use female German landrace pigs (or comparable) for this protocol. Figure 1 depicts the summary of the described experimental protocol. Deliver the animals to the research facility 14 days before the surgery for acclimatization and house them in a temperature- and humidity-controlled barrier environment with a 12 h light and dark cycle (Figure 2). Fast the animals overnight before surgery.
  2. Premedicate ....

Representative Results

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Figure 1: Study flowchart and protocol.

Abbreviations used: POD-postoperative day; ECG-electrocardiography.

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Figure 2: Animal housing facility with real-time and continuous telemetry monitoring of up to 6 animals.

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