Double-Staining Method: A Technique to Visualize Plant-Fungus Interactions Using Ruthenium Red and Lactophenol Cotton Blue

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Overview

This video demonstrates a double staining method using ruthenium red and lactophenol cotton blue to visualize pectins and chitins during plant-fungus interactions in a coffee rust plant. The procedure helps understand plant defense mechanisms during fungal infections.

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Source: Marques, J. P. R., and Nuevo, L. G. Double-Staining Method to Detect Pectin in Plant-Fungus Interaction. J. Vis. Exp. (2022).

Double-Staining Method: A Technique to Visualize Plant-Fungus Interactions Using Ruthenium Red and Lactophenol Cotton Blue

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Overview