A subscription to JoVE is required to view this content. Sign in or start your free trial.
A Technique to Generate an Adjuvant-Induced Arthritis Mouse Model
In This Article
Overview
This video demonstrates a method to generate adjuvant-induced arthritis in a murine model. Complete Freund's adjuvant (CFA) is injected in the mouse ankle joint and periarticular areas. CFA activates the immune system, leading to neutrophil infiltration and exaggerated inflammation.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Induction of the adjuvant-induced arthritis (AA) mouse model
- Suspend complete Freund's adjuvant (CFA) by vortexing at least 5 s, then draw 100 µL of suspension into an insulin injector.
NOTE: We recommend using a completely new CFA in experiments to ensure sterility. - Anesthetize mice as described.
- Mark the chosen paw and inject 20 µL of CFA into the ankle joint space. Inject 20 µL of suspension into four ....
Representative Results
Figure 1: Representative results of the adjuvant-induced arthritis (AA) mouse model. (A) The ankle joint diameter was measured using a pocket thickness gauge. (B) Joint swelling was assessed based on the ankle joint diameter (n = 7). (C) Pictures of each arthritis score. (D) The severity of arthritis was graded on a scale of 0.......
Copyright © 2025 MyJoVE Corporation. All rights reserved
We use cookies to enhance your experience on our website.
By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.