eoe sub articles

EoE Sub Articles

1. Spray Inoculation with a Suspension of M. grisea Conidia
<ol>
	<li>Fungal culture for M.grisea
	<ol>
		<li>Prepare the oatmeal tomato agar (OTA) culture medium for fungal strains.</li>
		<li>Weigh 30 - 50 g of oatmeal, add this to 800 mL of distilled/deionized water (ddH2O), and boil the mixture for 30 min in the electric pot.</li>
		<li>Filter the boiled oatmeal juice into the beaker through a piece of gauze.</li>
		<li>Add 150 mL of tomato juice and 20...

a spray inoculation technique to establish fungal infection in rice plant leaves

Source: Zhang, M., et al. <a href="https://app.jove.com/v/57675">The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea.</a> J. Vis. Exp. (2018).
This video demonstrates a technique to establish the pathogenic fungus Magnaporthe grisea infection in rice plant leaves. The fungal conidia suspension is sprayed on sections of rice leaf, and the disease progression is assessed by scoring the formation of necrotic lesions.

A Spray Inoculation Technique to Establish Fungal Infection in Rice Plant Leaves

1. Spray Inoculation with a Suspension of M. grisea Conidia

	Fungal culture for M.grisea
	
		Prepare the oatmeal tomato agar (OTA) culture medium for ...

Take a Petri plate with sections of rice plant leaves positioned above a wet filter paper to prevent desiccation.
Take a suspension of conidia&#160; &#8212; reproductive spores &#8212; of the pathogenic fungus Magnaporthe grisea. Spray on the leaf sections and incubate under dark and humid conditions.
Moisture induces conidia to release mucilage, facilitating attachment to the leaf surface.
The conidia extend germ tubes, the tip of which differentiates into dome-shaped appressorium.
High solute concentration inside the appressorium generates turgor pressure, forming a penetration peg.
The small diameter of the peg focuses turgor-mediated force on a small leaf area, causing penetration into the leaf cell.
The peg differentiates into invasive hyphae to derive nutrition.
Incubate the leaf sections under fluorescent light for fungal growth.
The hyphae ramify within infected cells and invade neighboring cells through plasmodesmata.
The fungus secretes toxins to induce necrosis, resulting in leaf lesions. Score the lesions to assess infection severity.