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Intraduodenal Cannulation in a Rat Model for Immunopathology Studies

This video demonstrates intraduodenal cannulation in a rat model. After anesthetizing the rat, the duodenum is located and a small puncture is created. A J-shaped cannula with a beveled tip is carefully inserted and secured, and the incision is closed. The cannulated rat model enables intra-intestinal infusion for immunopathology studies.

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Preparations the Day Before the Surgical Procedure

  1. Fast the rat the night before surgery if required. Ensure the rat has free access to water.
  2. Prepare the solutions to be administered to the rat.
    1. Prepare a rehydration solution such as sterile saline or Ringers solution.
    2. Prepare anestheti.......

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Figure 1
Figure 1. Diagrammatic representation of the shape of the beveled tip of a carotid artery or lymph cannula, and the J-shaped beveled tip of a duodenal cannula.

Figure 2
Figure 2. Photographs of (A) the abdomen shaved in preparation to cannulate the lymp.......

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