eoe sub articles

EoE Sub Articles

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Serial Blood Collection and Plasma Monitoring for Exogenous Human Cytokine in Mice
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	<li>Blood collection via tail snip
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		<li>Disinfect the biosafety hood and assemble the mouse restrainer, scissors, and other tools/supplies for the procedure.</li>
		<li>Place the mouse in a re...

a mouse tail snip procedure for peripheral blood collection and plasma isolation

Source: Coats, J. S. et al., <a href="https://app.jove.com/v/55384">Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line.</a> J. Vis. Exp. (2017)
In this video, we demonstrate a tail-snipping procedure in a mouse model to collect peripheral blood. The collected blood is subsequently subjected to centrifugation to separate plasma, which can be used for further experiments.

A Mouse Tail Snip Procedure for Peripheral Blood Collection and Plasma Isolation

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Serial ...

Position a mouse within a disinfected chamber to ensure access to its tail.
Secure the chamber to minimize mouse movement.
Sterilize the tail and anesthetize it.
Using sharp surgical scissors, snip off a small portion from the tip of the mouse&#39;s tail, which contains a network of blood vessels.
This tail-snipping procedure enables the collection of a small volume of peripheral blood.
Collect the blood into a heparinized capillary tube using the tube&#39;s capillary action. The heparinized tube helps prevent blood coagulation.
Using a bulb syringe, transfer the blood into an EDTA-coated tube.
Invert the tube for proper mixing of the blood with EDTA, an anticoagulant. EDTA binds calcium ions within the blood, preventing coagulation.
Centrifuge the tube to separate the plasma from the blood cells.
Collect and transfer the plasma-containing top layer to a fresh tube.
Store the tube at lower temperatures to maintain the integrity of the plasma components.