eoe sub articles

EoE Sub Articles

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines
1. Blood Collection from Patients
CAUTION: Take appropriate laboratory safety measures when collecting whole blood from the patient. Wear gloves and glasses. If the patient is in isolation, please follow biosafety level 4 procedures.
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	<li>To collect intravenous whole blood from the patient, use a butterfly need...

a technique for magnetic glass particle-mediated nucleic acid extraction from blood

Source: Rosenstierne, M. W., et al. <a href="https://app.jove.com/v/58001">Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples.</a> J. Vis. Exp. (2018).
This video demonstrates a technique for nucleic acid extraction from whole human blood using magnetic glass particles (MGPs) &#8212; small magnetic beads coated with silica. Upon lysing the blood cells using a lysis buffer, the released nucleic acids bind to the surface of silica-coated MGPs, extracting the molecules from the sample.

A Technique for Magnetic Glass Particle-Mediated Nucleic Acid Extraction from Blood

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines
1. Blood Collection from Patients
...

Take a tube containing freshly drawn human blood and add a lysis buffer.
The detergent in the buffer permeabilizes the cell membrane, lysing the cells and releasing intracellular nucleic acids, or NAs, and nucleases.
Chaotropic agents in the buffer denature nucleases, preventing NA degradation.
Add magnetic glass particles or MGPs &#160;&#8212; silica-coated magnetic particles &#8212; and mix thoroughly.
The buffer&#39;s low pH protonates silica, rendering a positive charge on the MGPs&#39; surface.
Negatively charged NAs bind electrostatically to the charged surface, forming an NA-MGP complex.
Under a magnetic field, NA-MGP complexes collect at the side of the tube.
Discard the supernatant containing cell debris and proteins.
Perform multiple washing steps by resuspending the NA-MGP complexes in a wash buffer, applying a magnetic field to collect the complexes, and discarding the supernatant with the remaining contaminants.
Resuspend complexes in an elution buffer. The high pH disrupts NA-MGP interaction, releasing the NAs. The eluted NAs are ready for downstream assays.