JoVE Encyclopedia of Experiments
Encyclopedia of Experiments
This video demonstrates a technique for nucleic acid extraction from whole human blood using magnetic glass particles (MGPs) — small magnetic beads coated with silica. Upon lysing the blood cells using a lysis buffer, the released nucleic acids bind to the surface of silica-coated MGPs, extracting the molecules from the sample.
All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines
1. Blood Collection from Patients
CAUTION: Take appropriate laboratory safety measures when collecting whole blood from the patient. Wear gloves and glasses. If the patient is in isolation, please follow biosafety level 4 procedures.
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Source: Rosenstierne, M. W., et al. Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples. J. Vis. Exp. (2018).
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