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Real-Time Monitoring of Energy Metabolism in Human NK Cells Using an Extracellular Flux Analyzer

This video demonstrates the method to analyze energy metabolism in activated human NK cells using an extracellular flux analyzer. Different inhibitors of the electron transport chain complexes impact ATP synthesis, which is measured through the oxygen consumption rate or OCR, providing insights into cellular metabolism and bioenergetics.

All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines.

1. Reagent preparation

  1. Reagents for the isolation of natural killer (NK) cells
    NOTE: Prepare these reagents in a cell culture hood.
    1. Prepare NK separation buffer: Supplement phosphate-buffered saline, PBS (pH 7.4) with 1 mM ethylenediaminetetraacetic acid (EDTA) and 2% fetal calf serum (FCS) that h.......

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Source: Traba, J. et al., Analysis of Human Natural Killer Cell Metabolism. J. Vis. Exp. (2020)

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