Integrated Field Lysimetry and Porewater Sampling for Evaluation of Chemical Mobility in Soils and Established Vegetation

Summary

Field lysimetry and porewater sampling allow researchers to evaluate the fate of chemicals applied to soils and established vegetation. The goal of this protocol is to demonstrate how to install required instrumentation and collect samples for chemical analysis during integrated field lysimetry and porewater sampling experiments.

Abstract

Potentially toxic chemicals are routinely applied to land to meet growing demands on waste management and food production, but the fate of these chemicals is often not well understood. Here we demonstrate an integrated field lysimetry and porewater sampling method for evaluating the mobility of chemicals applied to soils and established vegetation. Lysimeters, open columns made of metal or plastic, are driven into bareground or vegetated soils. Porewater samplers, which are commercially available and use vacuum to collect percolating soil water, are installed at predetermined depths within the lysimeters. At prearranged times following chemical application to experimental plots, porewater is collected, and lysimeters, containing soil and vegetation, are exhumed. By analyzing chemical concentrations in the lysimeter soil, vegetation, and porewater, downward leaching rates, soil retention capacities, and plant uptake for the chemical of interest may be quantified. Because field lysimetry and porewater sampling are conducted under natural environmental conditions and with minimal soil disturbance, derived results project real-case scenarios and provide valuable information for chemical management. As chemicals are increasingly applied to land worldwide, the described techniques may be utilized to determine whether applied chemicals pose adverse effects to human health or the environment.

Introduction

Potentially toxic chemicals are routinely applied to land from sources such as pesticides, fertilizers, sewage/biosolids, industrial wastes, and municipal wastes^1,2. The fate of these chemicals – which may include nutrients, trace elements, organics, and their associated metabolites – is often not well understood³. If the chemicals are not managed properly, they have the potential to threaten human and environmental health through their transfer to and buildup in plants, surface water, and groundwater. With a global population that may reach 10 billion people by 2050, there are growing demands on waste management and food production², and land application of many chemicals has been increasing^3,4. Accordingly, research is needed that quantifies the transformations, mobility, loading limits, and overall environmental risks from chemicals that require land disposal or that we depend upon to enhance crop health and yield.

A number of strategies have been employed to evaluate threats from chemicals applied in the environment. Laboratory-based, model-system studies have been conducted to provide information about fundamental mechanisms controlling the mobility of chemicals in soils. When analyzing chemical fate in a laboratory, complete manipulation of the “environment” and inputs may be achieved, but these rarely match real-world environmental conditions^5,6. Thus, extrapolating lab results to field settings may lead to inaccurate predictions about chemical threats. In contrast, broad field measurements have been used to define chemical behavior in the environment. However, conclusions about environmental fate from these measurements are often complicated due to the frequently low use rates (e.g. a few g A^-1) of applied chemicals, as well as the complex interactions between hydrological and biogeochemical processes in the environment that regulate chemical distributions.

Lysimetry, including field lysimetry, has historically been used by soil and crop scientists to systematically evaluate the downward mobility of chemicals applied to soils and established vegetation. A lysimeter is a device made of metal or plastic that is placed into a soil of interest and is used to determine the fate of chemicals applied in known amounts to a confined area. Soil and vegetation samples collected from lysimeters can be used to assess the evolution of chemical distributions over time. Because field lysimetry is carried out under natural environmental conditions, results may be used to predict real-case scenarios derived from chemical applications to soil systems. Early lysimeter studies measured transpiration, moisture flow, and/or nutrient movement. Modern-day lysimeter studies measure pesticide and nutrient dissipation, pesticide movement, volatility, and mass balance, along with the aforementioned measurements³.

A limitation of traditional field lysimetry is that chemical mobility within a soil profile is largely defined by solid-phase measurements, while less attention is paid to dissolved chemical concentrations in water percolating through the soils – a critical component that may impact the potential for groundwater contamination from land-applied chemicals. Although leachate from the bottom of the lysimeters is sometimes collected for analysis, this approach limits depth resolution of porewater concentrations and typically requires significant soil excavation prior to experimentation. Instead, to obtain data about chemical concentrations in soil water, porewater samplers may be utilized in field settings. Porewater samplers are installed in soils to collect water from discrete, desired depths and only minimally disturb the soil system. Porewater samplers have been referred to by many names including lysimeters, suction cup lysimeters, or soil solution samplers, convoluting their distinction with the traditional field lysimeters described above. In this paper, we will use the term “porewater sampler” to alleviate confusion.

Here, we demonstrate an experimental approach that combines field lysimetry and porewater sampling for evaluating the downward leaching potential of chemicals applied to vegetated soil or bareground systems. Lysimetry has been a powerful tool used since the 1700s⁷, while ceramic porewater sampling has been used since the early 1960s⁸. Integration of these robust techniques allows for field determination of both solid- and dissolved-phase chemical concentration distributions while minimizing soil disturbance. This paper describes factors to consider when designing an experiment, including site selection, device installation, and sample collection. The approach is illustrated with an experiment that evaluated the fate of an organic arsenical pesticide applied to a bareground and an established turfgrass system. The techniques described can be adjusted as necessary to examine the fate of a wide variety of chemicals, thereby providing invaluable tools to researchers and policy-makers who seek to understand the environmental fate and behavior of land-applied chemicals.

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Protocol

Field sampling is performed in this experiment and is under the authorization of the North Carolina Department of Agriculture & Consumer Services.

1. Field Lysimeter Installation

1. Choose an experimental site on which lateral movement of applied chemicals is unlikely (i.e. sites with little or no slope). Select sites based on soil and vegetation properties of interest.
2. If plots are vegetated, pull vegetation plugs prior to lysimeter installation (Figure 1A).
3. Drive the lysimeters downward into the desired plots (with or without vegetation) using an inverted post driver, leaving ~1-2 cm of the lysimeter above the soil surface to contain the applied chemical and minimize lateral chemical movement. For this, use rolled and welded eighteen-gauge steel sheets (91 cm depth x 15 cm diameter) (Figure 1B). Use lysimeters of different materials and dimensions to fit research objectives.
4. Replace vegetation plugs following lysimeter installation.
5. Manage any vegetation as appropriate for the experiment. If plots are to remain bare, use spot applications of glyphosate to keep the areas free of vegetation.
6. Ensure that irrigation, fertilization, and any other management practices are identical in the bareground and vegetated plots. Predetermine irrigation to meet research objectives.

2. Porewater Sampler Installation

1. Install porewater samplers, such as PTFE/quartz (50/50%), in the middle of lysimeters to collect percolating porewater.
2. Place a 2.5 cm stainless steel rod in the center of the lysimeter and insert it into the ground with a mallet to the desired sampler depth.
   NOTE: An auger may also be used for this step.
3. Prepare a silica flour and water slurry with 700 ml of irrigation water to ~900 g of chemically inert silica flour. Mix the slurry thoroughly before each sampler is placed in the mixture. Apply pressure between -50 to -70 kPa to the sampler from a handheld or battery-powered vacuum pump.
4. Remove the sampler from the silica flour slurry after 10 min, and thoroughly mix the silica slurry again. Pour 60 ml of the slurry through a funnel connected to a 2.5 cm diameter pipe into the bottom of the hole.
5. Place the sampler in the hole at the desired sampling depth with a plastic or metal pipe. Ensure that tubing from the sampler extends out of the hole. Use a slurry of nontreated, native soil and water to backfill the remaining hole.
6. Allow time during backfilling for soil to settle; use a pipe to tamp added soil as needed.
7. Backfill soil to the original level. If appropriate, replace vegetation at the top of the hole.
8. Attach sampler tubing to a vacuum bottle via a section of fluorinated ethylene propylene (FEP) tubing. With a plastic tube clamp, connect a second tubing line out of the vacuum bottle to a vacuum pump.
9. Cover tubing and collection bottles with black plastic or tape if the chemical(s) of interest is prone to photodegradation (Figure 1C).
10. Apply vacuum pressure of approximately -50 to -70 kPa via the vacuum bottle to the samplers repeatedly over the course of several days before experimentation to ensure proper sampler installation.

3. Chemical Application to Lysimeters

1. Allow at least two weeks for acclimation before chemical applications are made.
2. Collect background porewater samples before lysimeter treatment to quantify background concentrations of the chemical(s) of interest.
3. Apply the chemical of interest to the soil or vegetation by typical methods, such as with a hand-held CO₂-pressurized boom sprayer (Figure 1D) or by distributing the granular formulation directly on the surface of the plot containing the lysimeter. If multiple chemical applications are necessary for effectiveness, apply them per typical use patterns or label directions. Leave some lysimeters untreated to serve as a control.

4. Porewater Collection and Analysis

1. Apply approximately -50 to -70 kPa of vacuum to the porewater sampler vacuum bottles the day before or the day of sampling. Water surrounding the sampler will be drawn up through the sampler into the tubing, flowing to the vacuum bottle where it is collected until sampled. The soil volume from which porewater is collected and the water collection time may depend on factors such as soil type, soil texture, soil moisture content, and sampler depth.
2. Collect samples at specified time intervals following chemical application, as predetermined by the researcher.
3. Measure the volume of water collected into a graduated cylinder for each porewater sampler. If filtration is necessary, place the water in a Luer-Lok syringe (size will depend on volume of water) and pass sample through a 25 mm 0.2 µm nylon filter.
4. If different sample preservation methods are required and sufficient sample is collected, divide the sample into unique containers.
5. Use a handheld pH meter to determine the pH of non-acidified samples.
6. Adjust the pH by adding an adequate volume of the appropriate acid if needed for sample preservation.
   NOTE: Concentrated acids can be corrosive or oxidizers and care should be taken when using them.
7. Place samples on ice in a cooler or put in a refrigerator until analysis. Use analytical methods for chemical measurement such as inductively coupled plasma-mass spectrometry (ICP-MS), inductively coupled plasma-optical emission spectroscopy (ICP-OES), atomic absorption spectroscopy (AAS), or high-performance liquid chromatography (HPLC) to analyze the samples.

5. Lysimeter Exhumation, Soil/Vegetation Collection and Analysis

1. Exhume the lysimeters, containing soil and vegetation, at specified time intervals following chemical application. Exhume nontreated lysimeters at each sampling time to determine background chemical concentrations within the soil and vegetation.
2. Exhume lysimeters utilizing barrel clamps attached to a tractor implement. Lower the bucket to a position which allows for the clamps to be placed onto the lysimeter’s exposed edge.
3. Lift the implement causing the clamps to grasp the exposed edge, pulling the lysimeter column out of the soil (Figure 1E).
4. Cap exhumed lysimeter ends with insulation sheets cut to the diameter of the lysimeters. Hold caps in place with gallon-size polyethylene bags inserted over the lysimeter ends, and secure bags with duct tape.
5. Transport the lysimeters to a field laboratory for soil and vegetation sample division. Process nontreated lysimeters first to prevent contamination among lysimeters.
6. Use a reciprocating saw equipped with a metal cutting blade to cut the lysimeter lengthwise on one side. Cut the columns from the bottom (zone of anticipated lower concentration) to top (zone of anticipated higher concentration) to ensure the soil at deeper depths is not contaminated by soil at shallower depths.
7. Split open the lysimeter. Use metal dividing plates to separate discrete soil and vegetation sections. Choose soil depth increments based on the length of the lysimeter and research objectives.
8. Use spoons or spatulas to excavate the sectioned soil and vegetation. Place each sample in an appropriately labeled polyethylene freezer bag. Do not collect soil directly in contact with the lysimeter.
9. Follow the excavation protocol for each desired sample depth. Place sample bags in a cooler filled with ice and transport them to a lab. Store samples in a freezer until analysis.

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Results

This method allows for the accumulation of data on the fate of chemicals applied to bareground and vegetated soil systems^5,10. This approach was used to evaluate arsenic (As) downward leaching, absorption, and translocation into plants for bermudagrass (Cynodon dactylon) systems following application of the organic arsenical herbicide monosodium methyl arsenate (MSMA)⁹. Since the 1960s, MSMA has been used in non-cropland, turfgrass, and cotton production, but there is growing concern that a...

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Discussion

Utilizing an integrated field lysimetry and porewater sampling approach allows researchers to assess spatial and temporal distributions of a wide variety of land-applied chemicals. The fate of chemicals in soils and vegetated systems may be controlled by a number of environmental processes and attributes, such as downward leaching, volatilization, hydrolysis, photolysis, microbial transformation/degradation, plant uptake, soil type, and soil pH^16,17. Unlike greenhouse or laboratory-based experiments, results f...

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Materials

Name	Company	Catalog Number	Comments
Prenart Super Quartz Samplers (PFTE/Quartz)	Prenart Equipment ApS	N/A	Any samplers for  trace metal analysis can be used (e.g. SoilMoisture Equipment Corp.)
Prenart installation kit	Prenart Equipment ApS	N/A	Contains all items necessary to install porewater samplers
2 L collecting bottles	Prenart Equipment ApS		Bottles can also be purchased from Fisher Scientific (02-923-2) or other laboratory supply companies, but fittings will need to be adjusted. Bottles can be covered with dark material if light sensitive
Portable vacuum pump	Prenart Equipment ApS	N/A	Vacuporter from Decagon Devices or other field battery-operated or hand vacuum pump may be used
1 oz HDPE Nalgene bottles	Fisher Scientific	03-313-4A	Sample bottle type will depend on analyte of interest and may be glass
Concentrated nitric acid	Fisher Scientific	A509-P212	Oxidizing and corrosive-other acids may be needed for preservation and should be used with caution
25 mm 0.2 µm nylon syringe filters	VWR	28145-487	Other filter types and pore sizes may be used, dependent on the analyte of interest and analytical instrumentation
60 ml Luer-Lok syringes	Fisher Scientific	13-689-8	Other sizes may be used depending on sample volume collected
Portable pH meter	VWR	248481-A01	Other pH meters can be used following calibration
Graduated cylinder	any	N/A
Field lysimeters (metal, plastic, etc.)	N/A	N/A	Often these are constructed based on the researchers specifications
Inverted post driver tractor	N/A	N/A	Any tractor can be used to install the lysimeters
Handheld boom sprayer	N/A	N/A	To apply the rate needed for application
Polyethylene bags	Johnson & Johnson	N/A	Other brands may be used for soil storage
Reciprocating saw	Black & Decker	N/A	Any reciprocating saw can be used with a metal cutting attachment