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In This Article

  • Summary
  • Abstract
  • Introduction
  • Protocol
  • Representative Results
  • Discussion
  • Acknowledgements
  • Materials
  • References
  • Reprints and Permissions

Summary

We introduce a semi-automatic protocol for shape analysis on brain structures, including image segmentation using open software, and further group-wise shape analysis using an automated modeling package. Here, we demonstrate each step of the 3D shape analysis protocol with hippocampal segmentation from brain MR images.

Abstract

Statistical shape analysis of brain structures has been used to investigate the association between their structural changes and pathological processes. We have developed a software package for accurate and robust shape modeling and group-wise analysis. Here, we introduce an pipeline for the shape analysis, from individual 3D shape modeling to quantitative group shape analysis. We also describe the pre-processing and segmentation steps using open software packages. This practical guide would help researchers save time and effort in 3D shape analysis on brain structures.

Introduction

Shape analysis of brain structures has emerged as the preferred tool to investigate their morphological changes under pathological processes, such as neurodegenerative diseases and aging1. Various computational methods are required to 1) accurately delineate the boundaries of target structures from medical images, 2) reconstruct the target shape in the form of 3D surface mesh, 3) build inter-subjects correspondence across the individual shape models via shape parameterization or surface registration, and 4) quantitatively assess the regional shape differences between individuals or groups. Over the past several years, many methods have been int....

Protocol

Brain MR images were acquired as per the protocol approved by the local institutional review board and ethics committee.

NOTE: The tools for shape modeling and analysis can be downloaded from the NITRC repository: https://www.nitrc.org/projects/dtmframework/. The GUI software (DTMModeling.exe) can be executed after extraction.

Representative Results

The shape modeling process described here has been employed for various neuroimaging studies on aging6,8,10 and Alzheimer's disease5,9. Especially, this shape modeling method showed its accuracy and sensitivity in the shape analysis on the hippocampus for an aging population of 654 subjects8. A quantitative ana.......

Discussion

In summary, we have described the software pipeline for the shape analysis on brain structures including (1) MR image segmentation using open tools (2) individual shape reconstruction using a deformable template model, and (3) quantitative shape difference measurement via transitive shape correspondence with the template model. Statistical analysis under the false discovery rate (FDR) correction is performed with the shape deformity to investigate the significance of morphological changes of brain structures, associated .......

Acknowledgements

The work was funded by the National Research Foundation of Korea (JP as the PI). JK is funded by Kyungpook National University Research Fund; and MCVH is funded by the Row Fogo Charitable Trust and the Royal Society of Edinburgh. The hippocampal segmentation was adapted from in-house guidelines written by Dr. Karen Ferguson, at the Centre for Clinical Brain Sciences, Edinburgh, UK.

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Materials

NameCompanyCatalog NumberComments

References

  1. Costafreda, S. G., et al. Automated hippocampal shape analysis predicts the onset of dementia in mild cognitive impairment. NeuroImage. 56 (1), 212-219 (2011).
  2. Platero, C., Lin, L., Tobar, M. C.

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3D Shape ModelingBrain StructuresAnatomical CorrespondenceShape DeformityHippocampal SegmentationGroup wise TemplateShape ReconstructionShape And Deformity Measurement

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