Abstract
Bioengineering
Subretinal Implantation of RPE on a Carrier in Minipigs: Guidelines for Preoperative Preparations, Surgical Techniques, and Postoperative Care
ERRATUM NOTICE
Important: There has been an erratum issued for this article. Read more …Degenerative disorders of the retina (including age-related macular degeneration), which originate primarily at or within the retinal pigmented epithelial (RPE) layer, lead to a progressive disorganization of the retinal anatomy and the deterioration of visual function. The substitution of damaged RPE cells (RPEs) with in vitro cultured RPE cells using a subretinal cell carrier has shown potential for re-establishing the anatomical structure of the outer retinal layers and is, therefore, being further studied. Here, we present the principles of a surgical technique that allows for the effective subretinal transplantation of a cell carrier with cultivated RPEs into minipigs. The surgeries were performed under general anesthesia and included a standard lens-sparing three-port pars plana vitrectomy (PPV), subretinal application of a balanced salt solution (BSS), a 2.7 mm retinotomy, implantation of a nanofibrous cell carrier into the subretinal space through an additional 3.0 mm sclerotomy, fluid-air exchange (FAX), silicone oil tamponade, and closure of all the sclerotomies. This surgical approach was used in 29 surgeries (18 animals) over the past 8 years with a success rate of 93.1%. Anatomic verification of the surgical placement was carried out using in vivo fundus imaging (fundus photography and optical coherence tomography). The recommended surgical steps for the subretinal implantation of RPEs on a carrier in minipig eyes can be used in future preclinical studies using large-eye animal models.
Erratum
Erratum: Subretinal Implantation of RPE on a Carrier in Minipigs: Guidelines for Preoperative Preparations, Surgical Techniques, and Postoperative CareAn erratum was issued for: Subretinal Implantation of RPE on a Carrier in Minipigs: Guidelines for Preoperative Preparations, Surgical Techniques, and Postoperative Care. The Authors section was updated from:
Lyubomyr Lytvynchuk*1,2
Zbynek Stranak*3, Hana Studenovska4
David Rais4
Štěpán Popelka4
Lucie Tichotová5,6
Yaroslav Nemesh5,6
Anastasiia Kolesnikova5,6
Ruslan Nyshchuk5,6
Anna Brymová5,6
Zdeňka Ellederová5
Jana Čížková5
Jana Juhásová5
Štefan Juhás5
Pavla Jendelová7
Richárd Nagymihály8
Igor Kozak9
Slaven Erceg10
Susanne Binder11
Brigitte Müller1
Knut Stieger1
Jan Motlik5
Goran Petrovski*8
Taras Ardan*5
1Eye Clinic, Department of Ophthalmology, University Hospital Giessen and Marburg GmbH
2Karl Landsteiner Institute for Retinal Research and Imaging
3Department of Ophthalmology, University Hospital Kralovske Vinohrady and Third Faculty of Medicine, Charles University in Prague
4Institute of Macromolecular Chemistry, Czech Academy of Sciences, 5Institute of Animal Physiology and Genetics, Czech Academy of Sciences
6Department of Cell Biology, Faculty of Science, Charles University
7Institute of Experimental Medicine, Czech Academy of Sciences
8Center for Eye Research, Department of Ophthalmology, Oslo University Hospital and Institute of Clinical Medicine, Faculty of Medicine, University of Oslo
9Moorfields Eye Hospitals UAE
10Stem Cell Therapies in Neurodegenerative Diseases Lab, Research Center “Principe Felipe”
11Eye Center Donaustadt, Department of Ophthalmology, Sigmund Freud University
* These authors contributed equally
to:
Lyubomyr Lytvynchuk*1,2
Zbynek Stranak*3, Hana Studenovska4
David Rais4
Štěpán Popelka4
Lucie Tichotová5,6
Yaroslav Nemesh5,6
Anastasiia Kolesnikova5,6
Ruslan Nyshchuk5,6
Anna Brymová5,6
Zdeňka Ellederová5
Jana Čížková5
Jana Juhásová5
Štefan Juhás5
Pavla Jendelová7
Richárd Nagymihály8
Igor Kozak9
Slaven Erceg10
Susanne Binder11
Brigitte Müller1
Knut Stieger1
Jan Motlik5
Goran Petrovski*8
Taras Ardan*5
1Department of Ophthalmology, Justus Liebig University, University Hospital Giessen and Marburg GmbH
2Karl Landsteiner Institute for Retinal Research and Imaging
3Department of Ophthalmology, University Hospital Kralovske Vinohrady and Third Faculty of Medicine, Charles University in Prague
4Institute of Macromolecular Chemistry, Czech Academy of Sciences, 5Institute of Animal Physiology and Genetics, Czech Academy of Sciences
6Department of Cell Biology, Faculty of Science, Charles University
7Institute of Experimental Medicine, Czech Academy of Sciences
8Center for Eye Research, Department of Ophthalmology, Oslo University Hospital and Institute of Clinical Medicine, Faculty of Medicine, University of Oslo
9Moorfields Eye Hospitals UAE
10Stem Cell Therapies in Neurodegenerative Diseases Lab, Research Center “Principe Felipe”
11Eye Center Donaustadt, Department of Ophthalmology, Sigmund Freud University
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