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Method Article
The Morris water maze is a well-accepted tool used to document the involvement of the hippocampus in a behavioral task.
The Morris water maze is widely used to study spatial memory and learning. Animals are placed in a pool of water that is colored opaque with powdered non-fat milk or non-toxic tempera paint, where they must swim to a hidden escape platform. Because they are in opaque water, the animals cannot see the platform, and cannot rely on scent to find the escape route. Instead, they must rely on external/extra-maze cues. As the animals become more familiar with the task, they are able to find the platform more quickly. Developed by Richard G. Morris in 1984, this paradigm has become one of the "gold standards" of behavioral neuroscience.
Setting up the water maze
Pre-training for the water maze
Water maze testing
The water maze task was development by Morris 5. This task can be altered in numerous ways to investigate working memory, reference memory and task strategy 6. The procedure described here contains two critical variables which represent a deviation from other versions of the water maze: pretraining, and testing on a single day.
Pretraining - the hippocampus is involved in spatial/relational memory 7-8. The water maze specifically tests spatial...
The authors have nothing to disclose.
JLN was supported by MH 68347.
Material Name | Type | Company | Catalogue Number | Comment |
---|---|---|---|---|
Name | Company | Catalog Number | Comments | |
SMART System | San Diego Instruments | This system is not a requirement – there are many other behavioral systems on the market. |
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