Clinton C. MacDonald

Reexamining the polyadenylation signal: were we wrong about AAUAAA?

The gene CSTF2T, encoding the human variant CstF-64 polyadenylation protein tauCstF-64, lacks introns and may be associated with male sterility.

The flowers that bloom in the spring: RNA processing and seasonal flowering.

Developmental distribution of the polyadenylation protein CstF-64 and the variant tauCstF-64 in mouse and rat testis.

The mRNA encoding tauCstF-64 is expressed ubiquitously in mouse tissues.

Polyadenylation proteins CstF-64 and tauCstF-64 exhibit differential binding affinities for RNA polymers.

Differences in polyadenylation site choice between somatic and male germ cells.

Alternative mRNA polyadenylation can potentially affect detection of gene expression by affymetrix genechip arrays.

Systematic variation in mRNA 3'-processing signals during mouse spermatogenesis.

Loss of polyadenylation protein tauCstF-64 causes spermatogenic defects and male infertility.

A family of splice variants of CstF-64 expressed in vertebrate nervous systems.

The hinge domain of the cleavage stimulation factor protein CstF-64 is essential for CstF-77 interaction, nuclear localization, and polyadenylation.

Infertility with impaired zona pellucida adhesion of spermatozoa from mice lacking TauCstF-64.

Spermatogenetic but not immunological defects in mice lacking the τCstF-64 polyadenylation protein.

Tissue-specific mechanisms of alternative polyadenylation: testis, brain, and beyond.

The τCstF-64 polyadenylation protein controls genome expression in testis.

Polyadenylation site-specific differences in the activity of the neuronal βCstF-64 protein in PC-12 cells.

Application of recombinant human leukemia inhibitory factor (LIF) produced in rice (Oryza sativa L.) for maintenance of mouse embryonic stem cells.

The stem-loop luciferase assay for polyadenylation (SLAP) method for determining CstF-64-dependent polyadenylation activity.

High-throughput sequencing of RNA isolated by cross-linking and immunoprecipitation (HITS-CLIP) to determine sites of binding of CstF-64 on nascent RNAs.

Human leukemia inhibitory factor produced by the ExpressTec method from rice (Oryza sativa L.) is active in human neural stem cells and mouse induced pluripotent stem cells.

CstF-64 supports pluripotency and regulates cell cycle progression in embryonic stem cells through histone 3' end processing.

CstF-64 is necessary for endoderm differentiation resulting in cardiomyocyte defects.