Cytoplamic Lysate Preparation from Cultured Human Cells
5:15
Cytoplasmic Lysate Preparation from Mouse Testis
6:53
Sucrose Gradient Preparation and Ultracentrifugation
9:21
Polysome Fractionation and Sample Collection
11:45
Analysis of the Fractions
12:27
Results
14:14
Conclusions
Transkript
The overall goal of this technique is to study engagement of indivdual mRNA's with polysomes during translation. Polysome profiling is a powerful technology to study mRNA engagement with ribosomes and polysomes. The technique is important for stud
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The overall goal of polysome profiling technique is analysis of translational activity of individual mRNAs or transcriptome mRNAs during protein synthesis. The method is important for studies of protein synthesis regulation, translation activation and repression in health and multiple human diseases.