Teruo Okano

Cartilage repair in transplanted scaffold-free chondrocyte sheets using a minipig model.

Temperature-modulated adsorption of poly(N-isopropylacrylamide)-grafted ferritin on solid substrate.

Pluripotent stem cell-engineered cell sheets reassembled with defined cardiovascular populations ameliorate reduction in infarct heart function through cardiomyocyte-mediated neovascularization.

High stability of thermoresponsive polymer-brush-grafted silica beads as chromatography matrices.

Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro.

Optical mechanical refinement of human amniotic membrane by dehydration and cross-linking.

Myoblast sheet can prevent the impairment of cardiac diastolic function and late remodeling after left ventricular restoration in ischemic cardiomyopathy.

Thermoresponsive poly(N-isopropylacrylamide)-based block copolymer coating for optimizing cell sheet fabrication.

Repair of articular cartilage defect with layered chondrocyte sheets and cultured synovial cells.

Prevention of esophageal stricture after endoscopic submucosal dissection using tissue-engineered cell sheets.

Simultaneous enhancement of cell proliferation and thermally induced harvest efficiency based on temperature-responsive cationic copolymer-grafted microcarriers.

Punch and spindle-shaped biopsies for collecting oral mucosal tissue for the fabrication of transplantable autologous epithelial cell sheets.

A polylactic acid non-woven nerve conduit for facial nerve regeneration in rats.

Profiling of extracellular matrix and cadherin family gene expression in mouse feeder layer cells: type VI collagen is a candidate molecule inducing the colony formation of epithelial cells.

Cell shape regulation based on hepatocyte sheet engineering technologies.

Liver tissue engineering utilizing hepatocytes propagated in mouse livers in vivo.

Human hepatocyte propagation system in the mouse livers: functional maintenance of the production of coagulation and anticoagulation factors.

Thermally responsive microcarriers with optimal poly(N-isopropylacrylamide) grafted density for facilitating cell adhesion/detachment in suspension culture.

Shear stress-dependent cell detachment from temperature-responsive cell culture surfaces in a microfluidic device.

Creation of human cardiac cell sheets using pluripotent stem cells.

Fabricated autologous epidermal cell sheets for the prevention of esophageal stricture after circumferential ESD in a porcine model.

Development of eczematous symptoms by the implanted breast prosthesis.

Controlled formation of heterotypic hepatic micro-organoids in anisotropic hydrogel microfibers for long-term preservation of liver-specific functions.

Feasibility, safety, and therapeutic efficacy of human induced pluripotent stem cell-derived cardiomyocyte sheets in a porcine ischemic cardiomyopathy model.

The regulation of epithelial cell proliferation and growth by IL-1 receptor antagonist.

[Regenerative medicine using cell-sheet engineering].

New isolation system for collecting living cells from tissue.

Cell sheet technology for regeneration of esophageal mucosa.

Spontaneous fibrosarcoma in an experimental aged Lewis rat.

Control of the formation of vascular networks in 3D tissue engineered constructs.

Network formation through active migration of human vascular endothelial cells in a multilayered skeletal myoblast sheet.

Concise review: cell therapy and tissue engineering for cardiovascular disease.

Bioengineered myocardium derived from induced pluripotent stem cells improves cardiac function and attenuates cardiac remodeling following chronic myocardial infarction in rats.

A novel closed cell culture device for fabrication of corneal epithelial cell sheets.

Fabrication of mouse embryonic stem cell-derived layered cardiac cell sheets using a bioreactor culture system.

Myocardial layer-specific effect of myoblast cell-sheet implantation evaluated by tissue strain imaging.

Tissue factor triggers procoagulation in transplanted mesenchymal stem cells leading to thromboembolism.

Semi-circular microgrooves to observe active movements of individual Navicula pavillardii cells.

Genetically modified adipose tissue-derived stem/stromal cells, using simian immunodeficiency virus-based lentiviral vectors, in the treatment of hemophilia B.

In vitro fabrication of functional three-dimensional tissues with perfusable blood vessels.

Scale-independent stiffness measurement of upper limbs with lymphedema by a circular compression.

Diverse functions of secreted frizzled-related proteins in the osteoblastogenesis of human multipotent mesenchymal stromal cells.

Thermally modulated cationic copolymer brush on monolithic silica rods for high-speed separation of acidic biomolecules.

Mycoplasma removal from cell culture using antimicrobial photodynamic therapy.

In vitro engineering of vascularized tissue surrogates.

Modulation of cell adhesion and detachment on thermo-responsive polymeric surfaces through the observation of surface dynamics.

Fabrication of transplantable corneal epithelial and oral mucosal epithelial cell sheets using a novel temperature-responsive closed culture device.

Transplanted fibroblast cell sheets promote migration of hepatic progenitor cells in the incised host liver in allogeneic rat model.

Switching of cell growth/detachment on heparin-functionalized thermoresponsive surface for rapid cell sheet fabrication and manipulation.

Cell sheet transplantation for heart tissue repair.

Hormone supplying renal cell sheet in vivo produced by tissue engineering technology.

Endothelial cell behavior inside myoblast sheets with different thickness.

Comparison of angiogenic potential between prevascular and non-prevascular layered adipose-derived stem cell-sheets in early post-transplanted period.

Promotion of mouse ameloblast proliferation by Lgr5 mediated integrin signaling.

Improvement of cardiac stem cell sheet therapy for chronic ischemic injury by adding endothelial progenitor cell transplantation: analysis of layer-specific regional cardiac function.

A molded hyaluronic acid gel as a micro-template for blood capillaries.

Vascularization in 3D tissue using cell sheet technology.

Simple suspension culture system of human iPS cells maintaining their pluripotency for cardiac cell sheet engineering.

Self-oscillating polymer brushes.

Significantly different proliferative potential of oral mucosal epithelial cells between six animal species.

The use of anisotropic cell sheets to control orientation during the self-organization of 3D muscle tissue.

Characterization of chondrocyte sheets prepared using a co-culture method with temperature-responsive culture inserts.

Cell sheet engineering and its application for periodontal regeneration.

Middle ear mucosal regeneration with three-dimensionally tissue-engineered autologous middle ear cell sheets in rabbit model.

Terminal-functionality effect of poly(N-isopropylacrylamide) brush surfaces on temperature-controlled cell adhesion/detachment.

Rapid fabricating technique for multi-layered human hepatic cell sheets by forceful contraction of the fibroblast monolayer.

A device for the rapid transfer/transplantation of living cell sheets with the absence of cell damage.

Heparin-functionalized thermoresponsive surface: a versatile cell culture substrate for regulating multivalent affinity binding with heparin-binding proteins by temperature changes.

Corneal regeneration by transplantation of corneal epithelial cell sheets fabricated with automated cell culture system in rabbit model.

Hydrophobized thermoresponsive copolymer brushes for cell separation by multistep temperature change.

Splitting culture medium by air-jet and rewetting for the assessment of the wettability of cultured epithelial cell surfaces.

Enhanced survival of transplanted human induced pluripotent stem cell-derived cardiomyocytes by the combination of cell sheets with the pedicled omental flap technique in a porcine heart.

Potential utility of cell sheets derived from the anterior cruciate ligament and synovium fabricated in temperature-responsive culture dishes.

Expression profiles of angiogenesis-related proteins in prevascular three-dimensional tissues using cell-sheet engineering.

Rate control of cell sheet recovery by incorporating hydrophilic pattern in thermoresponsive cell culture dish.

Vascularized versus nonvascularized island median nerve grafts in the facial nerve regeneration and functional recovery of rats for facial nerve reconstruction study.

Addition of mesenchymal stem cells enhances the therapeutic effects of skeletal myoblast cell-sheet transplantation in a rat ischemic cardiomyopathy model.

CCAAT/enhancer binding protein-mediated regulation of TGFβ receptor 2 expression determines the hepatoblast fate decision.

Latest status of the clinical and industrial applications of cell sheet engineering and regenerative medicine.

Articular cartilage regeneration using cell sheet technology.

A new age of regenerative medicine: fusion of tissue engineering and stem cell research.

An interview with Teruo Okano, PhD.

Peritoneal cell sheets composed of mesothelial cells and fibroblasts prevent intra-abdominal adhesion formation in a rat model.

Control of cell adhesion and detachment on Langmuir-Schaefer surface composed of dodecyl-terminated thermo-responsive polymers.

Blue-violet light-emitting diode irradiation in combination with hemostatic gelatin sponge (Spongel) application ameliorates immediate socket bleeding in patients taking warfarin.

Surface design of antibody-immobilized thermoresponsive cell culture dishes for recovering intact cells by low-temperature treatment.

Tissue engineering in periodontal tissue.

Functional tissue engineering of the liver and islets.

Chondrocyte differentiation of human endometrial gland-derived MSCs in layered cell sheets.

A novel cell-sheet technology that achieves durable factor VIII delivery in a mouse model of hemophilia A.

In vivo cell tracking by bioluminescence imaging after transplantation of bioengineered cell sheets to the knee joint.

Automatic fabrication of 3-dimensional tissues using cell sheet manipulator technique.

Micropatterning with a liquid crystal display (LCD) projector.

Improved enzymatic treatment for accurate cell counting from extracellular matrix-rich periodontal ligament cell sheets.

A role for c-Kit in the maintenance of undifferentiated human mesenchymal stromal cells.

Toward the development of bioengineered human three-dimensional vascularized cardiac tissue using cell sheet technology.

Thermoresponsive copolymer brushes possessing quaternary amine groups for strong anion-exchange chromatographic matrices.

In vivo vascularization of cell sheets provided better long-term tissue survival than injection of cell suspension.

Monolithic silica rods grafted with thermoresponsive anionic polymer brushes for high-speed separation of basic biomolecules and peptides.

Nov/CCN3 regulates long-term repopulating activity of murine hematopoietic stem cells via integrin αvβ3.

Reconstruction of functional endometrium-like tissue in vitro and in vivo using cell sheet engineering.

Facile cell sheet manipulation and transplantation by using in situ gelation method.

Transplantation of cancerous cell sheets effectively generates tumour-bearing model mice.

Endothelial colony-forming cells for preparing prevascular three-dimensional cell-dense tissues using cell-sheet engineering.

Regenerative medicine: tissue-engineered cell sheet for the prevention of post-esophageal ESD stricture.

Accelerated cell-sheet recovery from a surface successively grafted with polyacrylamide and poly(N-isopropylacrylamide).

Cell/tissue processing information system for regenerative medicine.

Endothelial cells enhance the in vivo bone-forming ability of osteogenic cell sheets.

Facial nerve regeneration using basic fibroblast growth factor-impregnated gelatin microspheres in a rat model.

An immunocompetent, orthotopic mouse model of epithelial ovarian cancer utilizing tissue engineered tumor cell sheets.

Evidence of the survival of ectopically transplanted oral mucosal epithelial stem cells after repeated wounding of cornea.

Netrin-4 derived from murine vascular endothelial cells inhibits osteoclast differentiation in vitro and prevents bone loss in vivo.

Cell sheet approach for tissue engineering and regenerative medicine.

Undifferentiated and differentiated adipose-derived stem cells improve nerve regeneration in a rat model of facial nerve defect.

Cell sheet engineering for regenerative medicine: current challenges and strategies.

Polymeric micelles with stimuli-triggering systems for advanced cancer drug targeting.

Temperature-responsive polymeric micelles for optimizing drug targeting to solid tumors.

Cell sheet technology for cardiac tissue engineering.

Anisotropic cellular network formation in engineered muscle tissue through the self-organization of neurons and endothelial cells.

Application of regenerative medical technology using tissue-engineered cell sheets for endoscopic submucosal dissection of esophageal neoplasms.

Therapeutic applications of mesothelial cell sheets.

Thermoresponsive anionic copolymer brushes containing strong acid moieties for effective separation of basic biomolecules and proteins.

Stripe-patterned thermo-responsive cell culture dish for cell separation without cell labeling.

Micro-patterned cell-sheets fabricated with stamping-force-controlled micro-contact printing.

Elimination of remaining undifferentiated induced pluripotent stem cells in the process of human cardiac cell sheet fabrication using a methionine-free culture condition.

Thermoresponsive nanostructured surfaces generated by the Langmuir-Schaefer method are suitable for cell sheet fabrication.

Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice.

Ultrastructural features of ischemic tissue following application of a bio-membrane based progenitor cardiomyocyte patch for myocardial infarction repair.

Cell sheets engineering for esophageal regenerative medicine.

Human iPS cell-engineered cardiac tissue sheets with cardiomyocytes and vascular cells for cardiac regeneration.

Electrophysiologic and functional evaluations of regenerated facial nerve defects with a tube containing dental pulp cells in rats.

20. Multi-targeting system with fusion between thermoresponsive carriers and mild hyperthermia: Original research article: Thermo-responsive drug delivery from polymeric micelles constructed using block copolymers of poly(N-isopropylacrylamide) and poly(butylmethacrylate) (1999).

Prevention of esophageal strictures after endoscopic submucosal dissection.

Repair mechanism of osteochondral defect promoted by bioengineered chondrocyte sheet.

Cell-sheet therapy with omentopexy promotes arteriogenesis and improves coronary circulation physiology in failing heart.

Preventive effect of oral mucosal epithelial cell sheets on intrauterine adhesions.

[Regenerative medicine by cell sheet technology: the regeneration for stratified epithelial tissues].

'Erratum to "Micro-patterned cell-sheets fabricated with stamping-force-controlled micro-contact printing" [Biomaterials 35 (2014) 9802-10]'.

Thermoresponsive cationic copolymer brushes for mesenchymal stem cell separation.

Construction of three-dimensional vascularized cardiac tissue with cell sheet engineering.

Remodeling of epithelial cells and basement membranes in a corneal deficiency model with long-term follow-up.

The effect of transplantation of nasal mucosal epithelial cell sheets after middle ear surgery in a rabbit model.

Adipose-derived stem cell sheet transplantation therapy in a porcine model of chronic heart failure.

Three-dimensional functional human myocardial tissues fabricated from induced pluripotent stem cells.

Use of a microchamber for analysis of thermal variation of the gliding phenomenon of single Navicula pavillardii cells.

Engineered small diameter vascular grafts by combining cell sheet engineering and electrospinning technology.

A chemically defined culture medium containing Rho kinase inhibitor Y-27632 for the fabrication of stratified squamous epithelial cell grafts.

Allogeneic Transplantation of an Adipose-Derived Stem Cell Sheet Combined With Artificial Skin Accelerates Wound Healing in a Rat Wound Model of Type 2 Diabetes and Obesity.

Autologous human nasal epithelial cell sheet using temperature-responsive culture insert for transplantation after middle ear surgery.

Endoscopic cell sheet transplantation device developed by using a 3-dimensional printer and its feasibility evaluation in a porcine model.

Novel isolated cecal pouch model for endoscopic observation in rats.

Cell Sheet-Based Tissue Engineering for Organizing Anisotropic Tissue Constructs Produced Using Microfabricated Thermoresponsive Substrates.

Xenotransplantation of Bone Marrow-Derived Human Mesenchymal Stem Cell Sheets Attenuates Left Ventricular Remodeling in a Porcine Ischemic Cardiomyopathy Model.

Re-examination of regulatory opinions in Europe: possible contribution for the approval of the first gene therapy product Glybera.

A Method for Performing Islet Transplantation Using Tissue-Engineered Sheets of Islets and Mesenchymal Stem Cells.

Rapid fabrication system for three-dimensional tissues using cell sheet engineering and centrifugation.

Ex Vivo Prefabricated Rat Skin Flap Using Cell Sheets and an Arteriovenous Vascular Bundle.

Membrane-Permeable Calpain Inhibitors Promote Rat Oral Mucosal Epithelial Cell Proliferation by Inhibiting IL-1α Signaling.

Enhanced Wettability Changes by Synergistic Effect of Micro/Nanoimprinted Substrates and Grafted Thermoresponsive Polymer Brushes.

Time Course of Cell Sheet Adhesion to Porcine Heart Tissue after Transplantation.

Controlling shape and position of vascular formation in engineered tissues by arbitrary assembly of endothelial cells.

Characterization of rabbit limbal epithelial side population cells using RNA sequencing and single-cell qRT-PCR.

Fabrication of functional 3D hepatic tissues with polarized hepatocytes by stacking endothelial cell sheets in vitro.

Human mesenchymal stem cell-engineered hepatic cell sheets accelerate liver regeneration in mice.

Assessment of the Safety of Chondrocyte Sheet Implantation for Cartilage Regeneration.

A Biomimicking Tumor Tissue Model Using Hepatocellular Carcinoma Cell Sheet in a Collagen Sandwich System.

Temperature-responsive molecular recognition chromatography using phenylalanine and tryptophan derived polymer modified silica beads.

Tracing behavior of endothelial cells promotes vascular network formation.

Massively-multicellular alignment with the self-aggregate of air bubbles.

Recent development of temperature-responsive surfaces and their application for cell sheet engineering.

Quality of Air-Transported Human Islets for Single Islet Cell Preparations.

Autologous Skeletal Myoblast Sheet Therapy for Porcine Myocardial Infarction Without Increasing Risk of Arrhythmia.

Efficient Gene Transduction of Dispersed Islet Cells in Culture Using Fiber-Modified Adenoviral Vectors.

Human Laminin Isotype Coating for Creating Islet Cell Sheets.

Allogeneic Transplantation of Periodontal Ligament-Derived Multipotent Mesenchymal Stromal Cell Sheets in Canine Critical-Size Supra-Alveolar Periodontal Defect Model.

TRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues.

Retraction: Reproducible subcutaneous transplantation of cell sheets into recipient mice.

Multipotent mesenchymal stromal cell sheet therapy for bisphosphonate-related osteonecrosis of the jaw in a rat model.

The effect of tendon stem/progenitor cell (TSC) sheet on the early tendon healing in a rat Achilles tendon injury model.

Human Neural Tissue Construct Fabrication Based on Scaffold-Free Tissue Engineering.

ZBTB16 as a Downstream Target Gene of Osterix Regulates Osteoblastogenesis of Human Multipotent Mesenchymal Stromal Cells.

Characterization of layered chondrocyte sheets created in a co-culture system with synoviocytes in a hypoxic environment.

Artificial cilia as autonomous nanoactuators: Design of a gradient self-oscillating polymer brush with controlled unidirectional motion.

Treatment of chemically induced oral ulcer using adipose-derived mesenchymal stem cell sheet.

Design of Tetra-arm PEG-crosslinked Thermoresponsive Hydrogel for 3D Cell Culture.

Hepatocyte Is a Sole Cell Type Responsible for the Production of Coagulation Factor IX In Vivo.

Cell Therapy Using Adipose-Derived Stem Cells for Chronic Liver Injury in Mice.

Thicker three-dimensional tissue from a "symbiotic recycling system" combining mammalian cells and algae.

Adipose tissue-derived multi-lineage progenitor cells improve left ventricular dysfunction in porcine ischemic cardiomyopathy model.

Platelet-activated serum might have a therapeutic effect on damaged articular cartilage.

The effects of using vitrified chondrocyte sheets on pain alleviation and articular cartilage repair.

Thermoresponsive polymer-modified microfibers for cell separations.

On-off affinity binding modulation on thermoresponsive polymer-grafted surfaces for capture and release of proteins and cells.

Diverse Applications of Nanomedicine.

Production of pancreatic progenitor cells from human induced pluripotent stem cells using a three-dimensional suspension bioreactor system.

Fabrication of Micropatterned Self-Oscillating Polymer Brush for Direction Control of Chemical Waves.

Transplantation of tissue-engineered cell sheets for stricture prevention after endoscopic submucosal dissection of the oesophagus.

Local Release of VEGF Using Fiber Mats Enables Effective Transplantation of Layered Cardiomyocyte Sheets.

Functional Thyroid Follicular Cells Differentiation from Human-Induced Pluripotent Stem Cells in Suspension Culture.