in-vivo-monitoring-circadian-clock-gene-expression-mouse

<em>In Vivo</em> Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

This technique combines optical fiber mediated fluorescence recordings with the precise delivery of recombinant adeno-associated virus based gene ...

Peking University

National Institute of Biological Sciences, Beijing

Endogenous circadian clocks control 24-h physiological and behavioral rhythms in mammals. Here, we report a real-time in vivo fluorescence recording system that enables long-term monitoring of circadian rhythms in the brains of freely moving mice. With a designed reporter of circadian clock gene expression, we tracked robust  transcription reporter rhythms in the suprachiasmatic nucleus (SCN) of WT,  , and  mice in LD (12 h light, 12 h dark) and DD (constant darkness) conditions and verified that signals remained stable for over 6 mo. Further, we recorded  transcriptional rhythms in the subparaventricular zone (SPZ) and hippocampal CA1/2 regions of WT mice housed under LD and DD conditions. By using a Cre-loxP system, we recorded  and  transcription rhythms specifically in vasoactive intestinal peptide (VIP) neurons of the SCN. Finally, we demonstrated the dynamics of  and  transcriptional rhythms in SCN VIP neurons following an 8-h phase advance in the light/dark cycle.

Long Mei

PTN Joint Graduate Program,
School of Life Sciences,
PTN Joint Graduate Program, School of Life Sciences

Peking University

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

Long Mei

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Peking University

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

PTN Joint Graduate Program,
School of Life Sciences,
PTN Joint Graduate Program, School of Life Sciences