crispr-mediated-reorganization-of-chromatin-loop-structure

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Recent studies have clearly shown that long-range, three-dimensional chromatin looping interactions play a significant role in the regulation of gene ...

Stanford University School of Medicine

Alopecia areata (AA) is an autoimmune disease characterized by hair loss mediated by CD8 T cells. There are no reliably effective therapies for AA. Based on recent developments in the understanding of the pathomechanism of AA, JAK inhibitors appear to be a therapeutic option; however, their efficacy for the treatment of AA has not been systematically examined.

Safety and efficacy of the JAK inhibitor tofacitinib citrate in patients with alopecia areata.

Chromatin looping is key to gene regulation, yet no broadly applicable methods to selectively modify chromatin loops have been described. We have engineered a method for chromatin loop reorganization using CRISPR-dCas9 (CLOuD9) to selectively and reversibly establish chromatin loops. We demonstrate the power of this technology to selectively modulate gene expression at targeted loci.

Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping.

Hedgehog pathway-dependent cancers can escape Smoothened (SMO) inhibition through mutations in genes encoding canonical hedgehog pathway components; however, around 50% of drug-resistant basal cell carcinomas (BCCs) lack additional variants of these genes. Here we use multidimensional genomics analysis of human and mouse drug-resistant BCCs to identify a noncanonical hedgehog activation pathway driven by the transcription factor serum response factor (SRF). Active SRF along with its coactivator megakaryoblastic leukemia 1 (MKL1) binds DNA near hedgehog target genes and forms a previously unknown protein complex with the hedgehog transcription factor glioma-associated oncogene family zinc finger-1 (GLI1), causing amplification of GLI1 transcriptional activity. We show that cytoskeletal activation through Rho and the formin family member Diaphanous (mDia) is required for SRF-MKL-driven GLI1 activation and for tumor cell viability. Remarkably, nuclear MKL1 staining served as a biomarker in tumors from mice and human subjects to predict tumor responsiveness to MKL inhibitors, highlighting the therapeutic potential of targeting this pathway. Thus, our study illuminates, for the first time, cytoskeletal-activation-driven transcription as a personalized therapeutic target for combatting drug-resistant malignancies.

Gautam Shankar

Department of Dermatology,
Program in Epithelial Biology,
Department of Dermatology, Program in Epithelial Biology

Stanford University School of Medicine

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Gautam Shankar

Department of Dermatology,Program in Epithelial Biology,Department of Dermatology, Program in Epithelial Biology

Stanford University School of Medicine

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Department of Dermatology,
Program in Epithelial Biology,
Department of Dermatology, Program in Epithelial Biology