isolation-profiling-human-primary-mesenteric-arterial-endothelial

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Endothelial cells (ECs) are crucial for vascular and whole-body function through their dynamic response to environmental cues. Elucidating the ...

isolation-of-mouse-pancreatic-endothelial-cells

Isolation of Mouse Pancreatic Endothelial Cells

The pancreas is a vital organ for maintaining metabolic balance within the body, in part due to its production of metabolic hormones such as insulin and ...

City of Hope

The gut, with its extensive microbiota, plays a fundamental role in metabolism. While alterations of the gut microbiota can induce dysfunction of the endothelium, it remains unclear whether the endothelium can directly impact the gut microbiota. To answer this question, in this issue of EMBO Reports Haywood and colleagues deployed a murine model with endothelial-specific overexpression of human insulin-like growth factor-1 receptor (IGF-1R), termed hIGFREO mice (Haywood et al, 2021). When fed a high-fat diet, hIGFREO mice gained less weight and adiposity, with improved glucose tolerance, as compared to their wild-type littermates. Such protection was attributed to the difference in gut microbiota, exemplified by an increase in the beneficial genus Akkermansia. Furthermore, depletion of microbiota through broad-spectrum antibiotics nullified the advantageous metabolic phenotype observed. Collectively, these findings demonstrate a novel communication axis between the endothelium and the gut wall, specifically through endothelial IGF-1R modulation of gut microbiota, that promotes whole body metabolic homeostasis.

Naseeb Kaur Malhi

Department of Diabetes Complications and Metabolism

City of Hope

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Isolation of Mouse Pancreatic Endothelial Cells

Naseeb Kaur Malhi

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Diabetes Complications and Metabolism

City of Hope

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Isolation of Mouse Pancreatic Endothelial Cells

Department of Diabetes Complications and Metabolism