City of Hope
2 ARTICLES PUBLISHED IN JoVE
Medicine
Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level
Naseeb Kaur Malhi *1, Yingjun Luo *1, Xiaofang Tang *1, Kiran Sriram 1,3, Riccardo Calandrelli 2, Sheng Zhong 2, Zhen Bouman Chen 1,3
1Department of Diabetes Complications and Metabolism, City of Hope, 2Department of Bioengineering, University of California San Diego, 3Irell and Manella Graduate School of Biological Sciences, City of Hope
The protocol describes the isolation, culture, and profiling of endothelial cells from human mesenteric artery. Additionally, a method is provided to prepare human artery for spatial transcriptomics. Proteomics, transcriptomics, and functional assays can be performed on isolated cells. This protocol can be repurposed for any medium- or large-size artery.
Biology
Isolation of Mouse Pancreatic Endothelial Cells
Alonso Tapia 1,2, Naseeb Kaur Malhi 2, Xuejing Liu 2, Muxi Chen 2, Zhen Bouman Chen 1,2
1Irell and Manella Graduate School, City of Hope, 2Department of Diabetes Complications and Metabolism, City of Hope
This protocol describes the isolation of mouse endothelial cells from whole pancreas.
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