John F. X. Diffley

Author's overview: identifying SARS-CoV-2 antiviral compounds.

Recruitment of Mcm10 to Sites of Replication Initiation Requires Direct Binding to the Minichromosome Maintenance (MCM) Complex.

On the road to replication.

MCM: one ring to rule them all.

Phosphopeptide binding by Sld3 links Dbf4-dependent kinase to MCM replicative helicase activation.

Chromatin Controls DNA Replication Origin Selection, Lagging-Strand Synthesis, and Replication Fork Rates.

How the Eukaryotic Replisome Achieves Rapid and Efficient DNA Replication.

CMG-Pol epsilon dynamics suggests a mechanism for the establishment of leading-strand synthesis in the eukaryotic replisome.

Cdt1 stabilizes an open MCM ring for helicase loading.

Bidirectional eukaryotic DNA replication is established by quasi-symmetrical helicase loading.

Cryo-EM structure of a licensed DNA replication origin.

The mechanism of eukaryotic CMG helicase activation.

Structure of DNA-CMG-Pol epsilon elucidates the roles of the non-catalytic polymerase modules in the eukaryotic replisome.

Rpd3L Contributes to the DNA Damage Sensitivity of Checkpoint Mutants.

Mechanism of head-to-head MCM double-hexamer formation revealed by cryo-EM.

Multiplex Cell Fate Tracking by Flow Cytometry.

DNA replication origins retain mobile licensing proteins.

Budding yeast Rap1, but not telomeric DNA, is inhibitory for multiple stages of DNA replication in vitro.

Unchecked nick ligation can promote localized genome re-replication.

An improved method for the incorporation of fluoromethyl ketones into solid phase peptide synthesis techniques.

Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of nsp13 helicase.

Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of nsp12/7/8 RNA-dependent RNA polymerase.

Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of nsp15 endoribonuclease.

Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of Nsp3 papain-like protease.

Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of nsp14/nsp10 exoribonuclease.

Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of Nsp5 main protease.

Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of Nsp14 RNA cap methyltransferase.

Eukaryotic DNA replication with purified budding yeast proteins.

Structural mechanism for the selective phosphorylation of DNA-loaded MCM double hexamers by the Dbf4-dependent kinase.

The Initiation of Eukaryotic DNA Replication.

Mechanism of replication origin melting nucleated by CMG helicase assembly.

A DNA replication fork-centric view of the budding yeast DNA damage response.

Cyclin E-induced replicative stress drives p53-dependent whole-genome duplication.

A replication fork determinant for the establishment of sister chromatid cohesion.

Nucleotide binding halts diffusion of the eukaryotic replicative helicase during activation.

A chromatinized origin reduces the mobility of ORC and MCM through interactions and spatial constraint.

Unwinding of a eukaryotic origin of replication visualized by cryo-EM.