The Baise City Scientific Research and Technology Development Program (20224128) supported this work. The authors thank Dr. Dev Sooranna of Imperial College London and YMUN for editing the manuscript. YYX and ZYW contributed equally to this study.

The Laboratory Animal Ethics Committee of the Youjiang Medical University for Nationalities approved the study protocol (approval number: 2022101502). Female C57BL/6 mice, aged 10-12 weeks, SPF class and body weights (22 &#177; 2) g, were housed in the SPF Class Animal Experiment Center of Youjiang Medical University for Nationalities. The experimental animals were maintained at a temperature of 24-26 &#176;C and a relative humidity of 55% to 60%.
1. Traditional Chinese medicine systems ...

Therapeutic Effects of Xiaoyao Pills on Postmenopausal Osteoporosis in Mice

The authors declare there is no conflict of interest.

According to statistics, osteoporosis causes 1.5 million fractures each year in the United States, and the vast majority of these occur in postmenopausal women27. With an increase in the aging population, it is predicted that the majority of the world&#39;s future hip fractures will occur in Asia and that by 2050, the total number of these worldwide will reach 8.2 million28. The cost of preventing fractures is almost equal to that of treating them, and the use of medication...

The World Health Organization (WHO) defines osteoporosis (OP) as a disease characterized by a decrease in bone mass and deterioration of the microarchitecture of bone tissue, leading to an increase in bone brittleness and, thus an increased risk of fracture1. The clinical significance of osteoporosis is that it can lead to fractures, which are associated with high mortality, morbidity, and economic costs2. Postmenopausal osteoporosis (PMOP) is caused by a decrease in estrogen levels in women after menopause, which leads to an increase in osteoclast activity, resulting in bone loss and destruction of bone microstructure. This often causes osteoporosis with a serious impact on health3. Current therapies for PMOP include estrogen replacement therapy, bisphosphonates, and parathyroid hormone, but they can have varying degrees of adverse effects, insufficient long-term compliance, and high costs4. Therefore, affordable herbal medicine is a viable alternative for a large proportion of the population.
Xiaoyao pills are included in the Chinese Pharmacopoeia5, and these contain eight herbal components, including Chai Hu, Angelica sinensis, White paeonia lactiflora, Atractylodes macrocephala, Poria cocos, Menthae Herba, licorice, and fresh ginger. All these herbs are known to be effective in detoxifying the liver and strengthening the spleen, nourishing the blood, and regulating the menstrual cycle, and the mixture has also been used for treating depression6. However, the role of Xiaoyao pills in osteoporosis is unclear.
Early studies have suggested that inflammation can lead to bone loss7, and that the decline in bone density associated with this process may be accelerated by menopause. In addition, there is a strong relationship between the development of osteoporosis and inflammation. The inflammatory factor, interleukin-17 (IL-17), is a pro-inflammatory factor secreted by Th17 cells, a subset of CD4+ T lymphocytes. These cells are associated with several chronic inflammatory conditions, and they play an important role in the development of bone destruction in rheumatoid arthritis8. Additionally, IL-17 stimulates nuclear factor-&#954; B ligand receptor activator (RANKL), which regulates osteoclastogenesis, leading to greater bone resorption than bone formation9. IL-17 stimulates the expression of other osteoclastogenic cytokines such as TNF&#945;, IL-1, IL-6, and IL-8. It has the ability to synergize with other inflammatory factors, making it an important inflammatory effector10.
Studies have also shown a&#160;link between Xiaoyao pills and inflammation. Shi et al.11 and Fang et al.12 have recently confirmed that Xiaoyao pills can reduce the levels of IL-6 and TNF-&#945;, respectively. In another study of metabolism-associated steatohepatitis, it was reported that Xiaoyao pills could upregulate the expression of propionic acid, which in turn inhibits the expression of TNF-&#945; and exerts an anti-inflammatory effect13. However, at present, it is not known whether Xiaoyao pills can regulate the development of PMOM by mediating an inflammatory response through IL-17, which was the aim of this study.
This study predicted the intersection of the targets of Xiaoyao pills and osteoporosis-related genes through network pharmacology and bioinformatics analysis and analyzed the intersecting genes for protein interactions, GO, and KEGG. Based on the predicted results, the expression of Act1 and IL-6, which are key proteins in the IL-17 signaling pathway, can be observed14,15, as well as the bone turnover markers alkaline phosphatase (ALP) and collagen type I (COL-1), to observe the therapeutic efficacy of the Xiaoyao pills in the PMOM mice model.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>1ml Sampler</td>
			<td>Guangxi Beilunhe Medical Industrial Group Co.</td>
			<td>JYQ001</td>
			<td>For anesthesia in mice</td>
		</tr>
		<tr>
			<td>4%polyformaldehyde</td>
			<td>Beijing Solarbio Science &#38; Technology Co.,Ltd.</td>
			<td>P1110</td>
			<td>For tissue fixation</td>
		</tr>
		<tr>
			<td>6-0 absorbable suture (angled needle)</td>
			<td>Shanghai Pudong Jinhuan Medical Supplies Co.</td>
			<td>HZX-06</td>
			<td>For postoperative suturing</td>
		</tr>
		<tr>
			<td>Absorbent cotton ball</td>
			<td>Winner</td>
			<td>MIANQIU-500g</td>
			<td>For sterilization and hemostasis</td>
		</tr>
		<tr>
			<td>Adhesive slides</td>
			<td>Jiangsu Shitai Experimental Equipment Co.</td>
			<td>188105</td>
			<td>For tissue sectioning</td>
		</tr>
		<tr>
			<td>Amobarbital</td>
			<td>Sigma Aldrich (Shanghai) Trading Co.</td>
			<td>A-020-1ML</td>
			<td>For anesthesia in mice</td>
		</tr>
		<tr>
			<td>Bluing Solution</td>
			<td>Beijing Solarbio Science &#38; Technology Co.,Ltd.</td>
			<td>G1866</td>
			<td>Blue coloration of the nuclei of cells after the action of hematoxylin differentiation solution</td>
		</tr>
		<tr>
			<td>C57BL/6 mice</td>
			<td>Beijing Vital River Laboratory Animal Technology Co., Ltd.</td>
			<td>(SCXK 2033-0063)</td>
			<td>For use in animal experiments</td>
		</tr>
		<tr>
			<td>Carbon steel surgical blades</td>
			<td>Premier Medical Equipment Co.</td>
			<td>SP239</td>
			<td>For mouse surgery</td>
		</tr>
		<tr>
			<td>CIKS/TRAF3IP2 Rabbit pAb</td>
			<td>BIOSS ANTIBODIES</td>
			<td>bs-6202R</td>
			<td>Binds to Act1 in tissues</td>
		</tr>
		<tr>
			<td>Cole&#39;s Hematoxylin Solution (For Conventional Stain)</td>
			<td>Beijing Solarbio Science &#38; Technology Co.,Ltd.</td>
			<td>G1140</td>
			<td>For staining paraffin sections</td>
		</tr>
		<tr>
			<td>Collagen Type I Polyclonal antibody</td>
			<td>proteintech</td>
			<td>14695-1-AP</td>
			<td>Binds to COL-1 in tissues</td>
		</tr>
		<tr>
			<td>DAB Substrate kit,20x</td>
			<td>Beijing Solarbio Science &#38; Technology Co.,Ltd.</td>
			<td>DA1010</td>
			<td>For tissue color development</td>
		</tr>
		<tr>
			<td>Disposable surgical sheet 50*60CM</td>
			<td>Nanchang Xuhui Medical Equipment Co.</td>
			<td>SP4529777</td>
			<td>For mouse surgery</td>
		</tr>
		<tr>
			<td>EDTA decalcification solution (pH 7.2)</td>
			<td>Beijing Solarbio Science &#38; Technology Co.,Ltd.</td>
			<td>E1171-500ml</td>
			<td>For tissue decalcification</td>
		</tr>
		<tr>
			<td>Enhanced Endogenous Peroxidase Bloching Buffer</td>
			<td>Beyotime Biotechnology</td>
			<td>P0100B</td>
			<td>Sequestration of tissue or cellular endogenous peroxidases</td>
		</tr>
		<tr>
			<td>Environmentally friendly dewaxing clear liquid</td>
			<td>Servicebio</td>
			<td>G1128-1L</td>
			<td>For dewaxing paraffin sections</td>
		</tr>
		<tr>
			<td>Ethyl Alcohol</td>
			<td>CHRON CHEMICALS</td>
			<td>64-17-5 (CAS)</td>
			<td>For dehydration of paraffin sections</td>
		</tr>
		<tr>
			<td>General Purpose Antibody Diluent</td>
			<td>Epizyme Biotech</td>
			<td>PS119L</td>
			<td>For antibody dilution</td>
		</tr>
		<tr>
			<td>Hematoxylin Differentiation Solution</td>
			<td>Servicebio</td>
			<td>G1039-500ML</td>
			<td>For differentiation after hematoxylin staining and removal of excessively bound and non-specifically adsorbed dye from tissues</td>
		</tr>
		<tr>
			<td>Hematoxylin Eosin (HE) Staining Kit</td>
			<td>Beijing Solarbio Science &#38; Technology Co.,Ltd.</td>
			<td>G1120-3*100ml</td>
			<td>For tissue staining</td>
		</tr>
		<tr>
			<td>High quality stainless steel surgical knife handle</td>
			<td>Premier Medical Equipment Co.</td>
			<td>SP0088</td>
			<td>For mouse surgery</td>
		</tr>
		<tr>
			<td>HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L)</td>
			<td>proteintech</td>
			<td>SA00001-2</td>
			<td>Binds to primary antibody and amplifies signal</td>
		</tr>
		<tr>
			<td>IL-17A Polyclonal antibody</td>
			<td>proteintech</td>
			<td>13082-1-AP</td>
			<td>Binds to IL-17 in tissues</td>
		</tr>
		<tr>
			<td>IL-6 Polyclonal antibody</td>
			<td>proteintech</td>
			<td>21865-1-AP</td>
			<td>Binds to IL-6 in tissues</td>
		</tr>
		<tr>
			<td>Immunohistochemistry pen</td>
			<td>Beijing Zhongshan Jinqiao Biotechnology Co.</td>
			<td>ZLI-9305 (YA0310)</td>
			<td>For drawing circles in immunohistochemistry</td>
		</tr>
		<tr>
			<td>Medical surgical suture Non-absorbent (ball) 5-0 3.5m</td>
			<td>Yangzhou Yuanlikang Medical Equipment Co.</td>
			<td>FHX-5-2</td>
			<td>For postoperative suturing</td>
		</tr>
		<tr>
			<td>Medical Suture Needles Angle Needles 4*10 3/8</td>
			<td>Chaohu Binxiong Medical Equipment Co.</td>
			<td>FHZ612-4</td>
			<td>For postoperative suturing</td>
		</tr>
		<tr>
			<td>Neutral Balsam</td>
			<td>Beijing Solarbio Science &#38; Technology Co.,Ltd.</td>
			<td>G8590</td>
			<td>As a slice sealer</td>
		</tr>
		<tr>
			<td>PBS(1X)</td>
			<td>Shenzhen Mohong Technology Co.,Ltd</td>
			<td>B0015</td>
			<td>Buffer for slide washing and partial solution dilution</td>
		</tr>
		<tr>
			<td>Protein Free Rapid Blocking Buffer (1X)</td>
			<td>Epizyme Biotech</td>
			<td>PS108P</td>
			<td>Avoiding non-specific binding of proteins</td>
		</tr>
		<tr>
			<td>Rabbit Anti-Bone Alkaline Phosphatase antibody</td>
			<td>BIOSS ANTIBODIES</td>
			<td>bs-6292R</td>
			<td>Binds to alkaline phosphatase in tissues</td>
		</tr>
		<tr>
			<td>Saline</td>
			<td>Affiliated Hospital Youjiang Medical University For Nationalities</td>
			<td>LHN500</td>
			<td>For animals by gavage</td>
		</tr>
		<tr>
			<td>Shaving/Electric clippers</td>
			<td>HANGZHOU HUAYUAN PET PRODUCTS CO., LTD.</td>
			<td>DTJ-002</td>
			<td>For shaving mice</td>
		</tr>
		<tr>
			<td>Stainless Steel Medical Needle Holder 14cm Coarse Needle</td>
			<td>Premier Medical Equipment Co.</td>
			<td>SP784</td>
			<td>For mouse surgery</td>
		</tr>
		<tr>
			<td>Stainless Steel Ophthalmic Forceps 10.5cm Curved (No Hook)</td>
			<td>Zhuoyouyue</td>
			<td>YKNWW-10.5</td>
			<td>For mouse surgery</td>
		</tr>
		<tr>
			<td>Stainless Steel Ophthalmic Scissors/Surgical Scissors 10CM Straight Tip</td>
			<td>Premier Medical Equipment Co.</td>
			<td>ZYJD-10-ZJ</td>
			<td>For mouse surgery</td>
		</tr>
		<tr>
			<td>Stainless Steel Tip Gastric Needle 12 Gauge 55mm Elbow</td>
			<td></td>
			<td>GWJ-12-55W</td>
			<td>For use in mice by gavage</td>
		</tr>
		<tr>
			<td>Tris-EDTA Antigen Repair Fluid (50x)</td>
			<td>proteintech</td>
			<td>PR30002</td>
			<td>For antigen repair of paraffin sections</td>
		</tr>
		<tr>
			<td>Wooden dissecting board 25*16cm</td>
			<td></td>
			<td>JP16*24</td>
			<td>For mouse surgery</td>
		</tr>
		<tr>
			<td>Xiaoyao pills</td>
			<td>Jiuzhitang Co.,Ltd.</td>
			<td>YPG-041</td>
			<td>For animal drug delivery</td>
		</tr>
		<tr>
			<td>Others</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>R 4.3.1</td>
			<td></td>
			<td></td>
			<td>Data processing</td>
		</tr>
		<tr>
			<td>Cytoscape3.9.1</td>
			<td>National Resource for Network Biology</td>
			<td></td>
			<td>Building a regulatory network for traditional Chinese medicine</td>
		</tr>
		<tr>
			<td>ImageJ 1.54f</td>
			<td>National Institutes of Health</td>
			<td></td>
			<td>Image processing for immunohistochemistry results</td>
		</tr>
		<tr>
			<td>Adobe Photoshop 24.0.0</td>
			<td>Adobe</td>
			<td></td>
			<td>For image combination</td>
		</tr>
		<tr>
			<td>GraphpadPrism 9.5</td>
			<td>GraphPad Software</td>
			<td></td>
			<td>Statistical analysis of data</td>
		</tr>
		<tr>
			<td>cellsens Dimension</td>
			<td>OLYMPUS</td>
			<td></td>
			<td>For slicing and photographing</td>
		</tr>
		<tr>
			<td>OLYMPUS BX53</td>
			<td>OLYMPUS</td>
			<td></td>
			<td>For HE staining and immunohistochemical section photography</td>
		</tr>
	</tbody>
</table>

studies on the anti-inflammatory effect of xiaoyao pills in the treatment of postmenopausal osteoporosis in mice

Osteoporosis is a common metabolic disease of elderly and postmenopausal women, with no obvious symptoms during its early stages. In the latter stages of this condition, the patients are prone to fractures, and this can seriously affect their health and quality of life. The worldwide increase in life expectancy has made osteoporosis a global concern. The Xiaoyao pills were previously 
used in the treatment of depression. In addition, the drug appeared to have estrogen-like activity, which affected the expression of ALP, an early osteoblast-specific marker, and COL-1, a major component of bone extracellular matrix. Xiaoyao pills were assessed for their effects on postmenopausal osteoporosis (PMOM) in mice. The target information of each herbal component of Xiaoyao pills was accessed through the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database. Information from GeneCards, OMIM, PharmGkb, TTD, DrugBank, and other websites was used to construct the regulatory network of the herbal complex through Cytoscape and String network to assess the protein interactions. Mice were ovariectomized, and treated with high and low doses of Xiaoyao pills and these were compared to controls. Their symptoms were assessed by immunocytochemistry of bone tissues. The results suggested that Xiaoyao pills had the ability to alleviate the symptoms of PMOM in ovariectomized mice through the IL-17 signaling pathway. This drug has the potential to become a novel therapeutic agent for the treatment of osteoporosis.

Author Spotlight: Integrating Traditional Chinese Medicine with Modern Pharmacology and Genomics for Assessing Postmenopausal Osteoporosis in Mice 

Studies on the Anti-Inflammatory Effect of Xiaoyao Pills in The Treatment of Postmenopausal Osteoporosis in Mice

The scope of this study was the therapeutic effect of the Chinese medicine, Xiaoyao pills on mice with postmenopausal osteoporosis. In this study, we wanted to confirm whether the Xiaoyao pills could exert a therapeutic effect and the specific mechanism. In this study, we combined the traditional Chinese medicine with modern pharmacology and genomics, and the arthritis bioinformatics to assert our Analyze the that we saw will show your peer.Also process compared with a single pharmacology or genomics. Analyze our scheme is more comprehensive and may provide a new idea for the treatments of osteoporosis. This idea will promote the development towards the field of traditional Chinese medicine for osteoporosis, promote an advanced understanding of the mechanism or traditional Chinese medicine.Its primary research idea and the direction of the provider, practical guidance and efficacy. Evaluation visit about clinical application.

Active ingredients and targets of action of Xiaoyao pills 
By searching the TCMSP database and screening according to the criteria of oral bioavailability (OB) &#8805; 30% and drug-like properties (DL) &#8805; 0.18, 125 active ingredients were found to exist in Xiaoyao pills. Among them, ingredients 6, 4, 9, 13, 2, 6, 83, and 2 were from White Paeonia lactiflora, Atractylodes macrocephala, Menthae herba, Chaihu, Angelica sinensis, Poria cocos, licorice an...

Postmenopausal osteoporosis has become a global public health problem. The aim of this study is to explore the therapeutic effects and related mechanisms of the traditional Chinese medicine Xiaoyao pills on this condition.

Osteoporosis is a common metabolic disease of elderly and postmenopausal women, with no obvious symptoms during its early stages. In the latter stages of ...

studies-on-anti-inflammatory-effect-xiaoyao-pills-treatment

Research

JoVE Journal

Biology

Creating Venn Diagrams and Protein-Protein Interaction Networks for Disease-Associated Genes and Drug Targets

To begin disease-associated gene merging, place the previously saved TXT files from five databases containing the herbal name, ingredient ID, gene ID, and the script Disease. R in the same folder. Open the R code.Copy and paste the path where the TXT file is stored into the line where the setwd is located in the R code. Open the R software. Run the modified R code, and Save.Set the gene and name the file Disease.txt. Open the Venn diagram website. In the Input section, copy and paste the already saved text content into the list.Name the content with the respective database. and click Submit. Click Save Image As PNG below the image and save the text result in the same folder.Place the alltargets.symbol. txt file and the disease. txt file in the same folder.Open the R code. Copy and paste the path where the txt file is stored into the line where the setwd is located In the R code. Open the R software.Run the modified R code, and Save. Set the gene and name the file Drug_Disease.txt. Open the Venn diagram website.In the Input section, copy and paste alltargets.symbol. txt and Disease. txt into the list and name the files.Click Save Image As PNG below the image, and save the text results in the same folder. Open the string website, and click Multiple protein. Click Browse and upload the DrugDisease.txt file. then select Homo sapiens in Organisms. Click SEARCH, followed by CONTINUE.Now, click Settings, and set the minimum required interaction score to the highest confidence. From the Network Display option, check Hide disconnected nodes in the network, then click UPDATE>Adjust the nodes in the network diagram so that there is no overlap or occlusion. Click Exports, followed by Download as a high resolution bitmap to get the Protein Interworking Network Map in PNG format, Download the TSV file as a short tabular text output.Save PNG and TSV files in a unified folder.

Immunohistochemical Analysis of Ovariectomized Mice Following Xiaoyao Pills Administration

To begin, prepare wax blocks from euthanized mice injected with Xiaoyao pills. Cut the wax blocks into six micrometer thick continuous sections, and bake the slices at 70 to 72 degrees Celsius for 30 to 60 minutes. Transfer the slices to the de-waxing solution for eight minutes, then dehydrate twice in 75%anhydrous ethanol for five minutes each before rinsing in PBS for five minutes.Now perform EDTA high pressure repair for 15 minutes. After rinsing with PBS, dropwise add 3%hydrogen peroxide to the tissue and incubate for 10 minutes. Wash the tissue three times with PBS for five minutes each.Using an immunohistochemistry pen, draw a circle around the tissue. After washing the tissue with PBS, stain overnight in primary antibody solution. The following day, rinse the tissue three times for five minutes each in PBS.Dropwise add the secondary antibody and incubate for 60 minutes. After rinsing with PBS, incubate the tissue in DAB ready-to-use solution for three to five minutes. Now stain the tissue in hematoxylin for three minutes.Again, rinse the tissue with PBS before placing it in differentiation solution for 10 seconds. After PBS rinse, add the return solution for 10 seconds. Finally, perform 75%anhydrous ethanol treatment for five minutes and seal the slide with neutral resin.Hematoxylin-eosin staining showed tightly-arranged trabeculae in sham mice, while OVX mice displayed typical osteoporotic changes with sparse trabeculae and enlarged marrow cavities. Micro CT comparison showed incomplete trabecular meshwork and thinning of the bone cortex in OVX mice compared to sham mice. After drug intervention, hematoxylin-eosin staining showed restored trabeculae structure and reduced marrow cavity compared to the OVX group.Imaging indices showed fewer fractured trabeculae and restored reticulation in the high-dose Xiaoyao pills group compared to the OVX group. Immunohistochemistry results showed increased alkaline phosphatase and collagen type one and decreased interleukin 17, ACT1, and interleukin 6 in the drug group.

233 Views.  Youjiang Medical University for Nationalities.  Postmenopausal osteoporosis has become a global public health problem. The aim of this study is to explore the therapeutic effects and related mechanisms of the traditional Chinese medicine Xiaoyao pills on this condition.

Therapeutic Effects of Xiaoyao Pills on Postmenopausal Osteoporosis in Mice

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