Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell ProteinsMarijke Grau 1, Lennart Kuck 2
1Department of Molecular and Cellular Sports Medicine, German Sport University Cologne, 2Biorheology Research Laboratory, Menzies Health Institute, Griffith University Gold Coast
Capturing dynamic changes in the protein activation of enucleated red blood cells poses methodological challenges, like the preservation of dynamic changes to acute stimuli for later assessment. The presented protocol describes sample preparation and staining techniques that enable preservation and analysis of relevant protein changes and subsequent detection.
