College of Charleston
2 ARTICLES PUBLISHED IN JoVE
Genetics
Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
Sophia Emetu *1, Morgan Troiano *1, Jacob Goldmintz *1, Jensen Tomberlin 1, Simon Grelet 2, Philip H. Howe 2, Christopher Korey 3, Renaud Geslain 1
1Laboratory of tRNA Biology, Department of Biology, College of Charleston, 2Department of Biochemistry and Molecular Biology, MUSC, 3Department of Biology, College of Charleston
We describe an original transfer RNA analysis platform named SPOt (Streamlined Platform for Observing tRNA). SPOt simultaneously measures cellular levels of all tRNAs in biological samples, in only three steps, and in less than 24 hours.
Education
Computation of Atmospheric Concentrations of Molecular Clusters from ab initio Thermochemistry
Tuguldur T. Odbadrakh 1, Ariel G. Gale 1, Benjamin T. Ball 1, Berhane Temelso 2, George C. Shields 1
1Department of Chemistry, Furman University, 2College of Charleston
The atmospheric concentrations of weakly bound molecular clusters can be computed from the thermochemical properties of low energy structures found through a multi-step configurational sampling methodology utilizing a genetic algorithm and semi-empirical and ab initio quantum chemistry.
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