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Washburn University

2 ARTICLES PUBLISHED IN JoVE

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Environment

Reliable Method for Assessing Seed Germination, Dormancy, and Mortality under Field Conditions
Brian A. Pace 1, Helen M. Alexander 2, D. Jason Emry 3, Kristin L. Mercer 1
1Department of Horticulture and Crop Science, Ohio State University, 2Department of Ecology and Evolutionary Biology, University of Kansas, 3Department of Biology, Washburn University

Here we present a protocol for assessing seed survivorship, germination and dormancy under field conditions using buried, labeled seed strips and tetrazolium chloride (TZ) viability testing.

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Biology

Transfection of a Molecular Clone of Naegleria gruberi rDNA into N. gruberi Trophozoites
Brian T. Nguyen 1, Nora M. Chapman 2, John C. Mullican 3, Kristen M. Drescher 1
1Department of Medical Microbiology and Immunology, Creighton University School of Medicine, 2Department of Pathology and Microbiology, University of Nebraska Medical Center, 3Department of Biology, Washburn University

This protocol describes a methodology to transfect Naegleria gruberi trophozoites with a construct that is maintained throughout passaging trophozoites in vitro, as well as through encystment and excystment.

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