New York University Langone Medical Center
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Design and Fabrication of Ultralight Weight, Adjustable Multi-electrode Probes for Electrophysiological Recordings in Mice
Philip M. Brunetti *1, Ralf D. Wimmer *1, Li Liang 1, Joshua H. Siegle 2, Jakob Voigts 2, Matthew Wilson 2, Michael M. Halassa 1
1The Neuroscience Institute, New York University Langone Medical Center, 2Department of Brain and Cognitive Science, Massachusetts Institute of Technology
Understanding the neural substrates of behavior requires brain circuit ensemble recording. Because of its genetic tractability, the mouse offers a model for circuit dissection and disease mimicry. Here, a method of designing and fabricating miniaturized probes is described that is suitable for targeting deep brain structure in the mouse.
Immunology and Infection
Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes
Saahir Khan 1, Aarti Jain 2, Omid Taghavian 2, Rie Nakajima 2, Algis Jasinskas 2, Medalyn Supnet 2, Jiin Felgner 2, Jenny Davies 2, Rafael Ramiro de Assis 2, Sharon Jan 2, Joshua Obiero 2, Erwin Strahsburger 2, Egest J. Pone 2, Li Liang 2, D. Huw Davies 2, Philip L. Felgner 2
1Division of Infectious Diseases, Department of Medicine, University of California Irvine Health, 2Vaccine Research and Development Center, Department of Physiology, University of California Irvine
We present a protocol for using a protein microarray constructed by printing influenza antigens onto nitrocellulose-coated slides to simultaneously probe serum for multiple antibody isotypes against over 250 antigens from different virus strains, thus allowing measurement of the breadth of serum antibodies across virus subtypes.
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