New York University Langone Medical Center

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Design and Fabrication of Ultralight Weight, Adjustable Multi-electrode Probes for Electrophysiological Recordings in Mice

Philip M. Brunetti^*1, Ralf D. Wimmer^*1, Li Liang¹, Joshua H. Siegle², Jakob Voigts², Matthew Wilson², Michael M. Halassa¹

¹The Neuroscience Institute, New York University Langone Medical Center, ²Department of Brain and Cognitive Science, Massachusetts Institute of Technology

Understanding the neural substrates of behavior requires brain circuit ensemble recording. Because of its genetic tractability, the mouse offers a model for circuit dissection and disease mimicry. Here, a method of designing and fabricating miniaturized probes is described that is suitable for targeting deep brain structure in the mouse.

Immunology and Infection

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

Saahir Khan¹, Aarti Jain², Omid Taghavian², Rie Nakajima², Algis Jasinskas², Medalyn Supnet², Jiin Felgner², Jenny Davies², Rafael Ramiro de Assis², Sharon Jan², Joshua Obiero², Erwin Strahsburger², Egest J. Pone², Li Liang², D. Huw Davies², Philip L. Felgner²

¹Division of Infectious Diseases, Department of Medicine, University of California Irvine Health, ²Vaccine Research and Development Center, Department of Physiology, University of California Irvine

We present a protocol for using a protein microarray constructed by printing influenza antigens onto nitrocellulose-coated slides to simultaneously probe serum for multiple antibody isotypes against over 250 antigens from different virus strains, thus allowing measurement of the breadth of serum antibodies across virus subtypes.