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University of Windsor

3 ARTICLES PUBLISHED IN JoVE

Medicine

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen

Dennis Ma¹, Jonathan Collins², Tomas Hudlicky², Siyaram Pandey¹

¹Department of Chemistry and Biochemistry, University of Windsor, ²Chemistry Department and Centre for Biotechnology, Brock University

We have synthesized a novel analogue of pancratistatin with comparable anti-cancer activity as native pancratistatin; interestingly, combinatory treatment with tamoxifen yielded a drastic enhancement in apoptotic and autophagic induction by mitochondrial targeting with minimal effect on noncancerous fibroblasts. Thus, JCTH-4 in combination with tamoxifen could provide a safe anti-cancer therapy.

Chemistry

Preparation and Reactivity of a Triphosphenium Bromide Salt: A Convenient and Stable Source of Phosphorus(I)

Stephanie C. Kosnik¹, Justin F. Binder¹, Maxemilian C. Nascimento¹, Charles L. B. Macdonald¹

¹Department of Chemistry and Biochemistry, University of Windsor

The synthesis of a triphosphenium bromide salt is described and its use as a P⁺ transfer agent is outlined by reactions with an N-heterocyclic carbene and an anionic bisphosphine, yielding an NHC-stabilized P(I) cation and a P(I) containing zwitterion, respectively.

Engineering

Measuring the Time-Evolution of Nanoscale Materials with Stopped-Flow and Small-Angle Neutron Scattering

Elizabeth G. Kelley¹, Michael H. L. Nguyen², Drew Marquardt^2,3, Brian B. Maranville¹, Ryan P. Murphy¹

¹NIST Center for Neutron Research, National Institute of Standards and Technology, ²Department of Chemistry and Biochemistry, University of Windsor, ³Department of Physics, University of Windsor

This protocol presents the use of a stopped-flow sample environment to quickly mix multiple liquid solutions in situ during a small-angle neutron scattering measurement and to study kinetic processes on nanometer length scales and second time scales.