University Hospital Cologne
2 ARTICLES PUBLISHED IN JoVE
Medicine
The Rabbit Blood-shunt Model for the Study of Acute and Late Sequelae of Subarachnoid Hemorrhage: Technical Aspects
Lukas Andereggen 3,4,5, Volker Neuschmelting 1,6, Michael von Gunten 7, Hans Rudolf Widmer 5, Jukka Takala 1, Stephan M. Jakob 1, Javier Fandino 1,2, Serge Marbacher 1,2
1Department of Intensive Care Medicine, University and Bern University Hospital (Inselspital), 2Department of Neurosurgery, Kantonsspital Aarau, 3Laboratories for Neuroscience Research in Neurosurgery, Boston Children's Hospital, 4Harvard Medical School, Boston Children's Hospital, 5Department of Neurosurgery, University and Bern University Hospital (Inselspital), 6Department of Neurosurgery, University Hospital Cologne, 7Institute of Pathology, Länggasse Bern
The experimental intracranial pressure-controlled blood shunt subarachnoid hemorrhage (SAH) model in the rabbit combines the standard procedures — subclavian artery cannulation and transcutaneous cisterna magna puncture, which enables close mimicking of human pathophysiological conditions after SAH. We present step-by-step instructions and discuss key surgical points for successful experimental SAH creation.
Cancer Research
Subculture and Cryopreservation of Esophageal Adenocarcinoma Organoids: Pros and Cons for Single Cell Digestion
Ningbo Fan *1, Lisa Raatz *1, Seung-Hun Chon 1, Alexander Quaas 2, Christiane Bruns 1, Yue Zhao 1
1Department of General, Visceral, Cancer and Transplantation Surgery, University Hospital Cologne, 2Institute of Pathology, University Hospital Cologne
This protocol describes the methods of subculture and cryopreservation of esophageal adenocarcinoma organoids with and without single cell digestion to enable researchers to choose appropriate strategies based on their experimental design.
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