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Arcadia University

4 ARTICLES PUBLISHED IN JoVE

Neuroscience

Subcutaneous Administration of Muscarinic Antagonists and Triple-Immunostaining of the Levator Auris Longus Muscle in Mice

Megan Wright¹, Amy Kim², Young-Jin Son³

¹Biology Department, Arcadia University, ²Shriners Hospitals Pediatric Research Center, Temple University School of Medicine, ³Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, Temple University School of Medicine

We describe procedures for repeated administration of inhibitors of muscarinic signaling to the levator auris longus (LAL) muscle of young adult mice and for subsequent immunostaining of its neuromuscular junctions (NMJs) in wholemounts. The LAL muscle has unique advantages for revealing in vivo pharmacological effects on NMJs.

Neuroscience

Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons

Melanie Martin^1,2, Ke Li¹, Megan C. Wright², Angelo C. Lepore¹

¹Department of Neuroscience, Farber Institute for Neurosciences, Sidney Kimmel Medical College at Thomas Jefferson University, ²Department of Biology, Arcadia University

Compound muscle action potential recording quantitatively assesses functional diaphragm innervation by phrenic motor neurons. Whole-mount diaphragm immunohistochemistry assesses morphological innervation at individual neuromuscular junctions. The goal of this protocol is to demonstrate how these two powerful methodologies can be used in various rodent models of spinal cord disease.

Environment

An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates

Trevor Cross¹, Courtney Schoff², Dylan Chudoff³, LIbby Graves³, Haley Broomell⁴, Katrina Terry⁴, Jennifer Farina⁵, Alexandra Correa⁵, David Shade⁵, David Dunbar⁵

¹Biology Department, University of the Sciences, ²Biology Department, Arcadia University, ³Biology Department, Immaculata University, ⁴Biology/Clinical Laboratory Science, Neumann University, ⁵Science Department, Cabrini College

We present an enrichment protocol for the isolation of bacteriophages infecting bacteria in the Arthrobacter genus. This enrichment protocol produces fast and reproducible results for the isolation and amplification of Arthrobacter phages from soil isolates.

Behavior

Using Rapid Serial Visual Presentation to Measure Set-Specific Capture, a Consequence of Distraction While Multitasking

Katherine S. Moore¹, Elizabeth A. Wiemers², Ariel Kershner¹, Korissa Belville¹, Jaimie Jasina¹, Aziza Ransome¹, Jessica Avanzato¹

¹Department of Psychology, Arcadia University, ²Department of Psychological Sciences, Purdue University

This method uses a dynamic visual display to index costs of distraction during visual search, including both "contingent attentional capture" and "set-specific capture," which is a cost of distraction that occurs when the participants maintain multiple search goals simultaneously. This method has revealed basic mechanisms and limitations of visual attention.