Tissue Processing and Isolation of Primary Fibroblasts from the Human VaginaVi Duong 1, Venkatesh Pooladanda 2, Caroline Mitchell 3
1Department of Obstetrics, Gynecology and Reproductive Biology, Division of Urogynecology, Massachusetts General Hospital, 2Massachusetts General Hospital/Vincent Center for Reproductive Biology/Harvard Medical School, 3Department of Obstetrics, Gynecology and Reproductive Biology, Massachusetts General Hospital, Vincent Center for Reproductive Biology
This protocol demonstrates a reliable and effective technique to isolate primary fibroblasts from either premenopausal or postmenopausal human vaginal tissue. Existing protocols for vaginal fibroblast isolation do not consider the challenges of cell isolation from senescent tissue. Vaginal tissue was obtained from women after pelvic organ prolapse surgery.
