Assessing Iron Deposition in the Brains of 5xFAD Mice by Perls'/DAB StainingShuning Sang *1, Chenhao Tian *1, Jiahui Ding 1,2, Suochen Pang 1,3, Chao Liu 1
1Jiangsu Province Key Laboratory of Anesthesiology, Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology, NMPA Key Laboratory for Research and Evaluation of Narcotic and Psychotropic Drugs, School of Anesthesiology, Xuzhou Medical University, 2Department of Anesthesiology, the Eye & ENT Hospital of Fudan University Shanghai, 3Department of Anesthesiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine
This protocol presents methods for assessing the distribution and quantity of iron deposition in tissues, especially the brain. The protocol details the procedures for sample preparation, Perls/DAB staining, image capture, and data analysis.
