Nova Southeastern University
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
Andrea Linares 1, Leena E. Couling 2, Eduardo J. Carrera 3, Robert C. Speth 2
1Farquhar College of Arts and Sciences, Nova Southeastern University, 2Department of Pharmaceutical Sciences, College of Pharmacy, Nova Southeastern University, 3School of Medicine, University of Colorado
Here we present a protocol to describe the localization of angiotensin II Type 1 receptors in the rat brain by quantitative, densitometric, in vitro receptor autoradiography using an iodine-125 labeled analog of angiotensin II.
Biology
Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models
Motoki Okamoto *1, Shohei Yamashita *1, Takumi Memida 1, Melanie Mendonca 1, Susanne Brueckner 1, Shin Nakamura 1, Satoru Shindo 1, Ria Achong-Bowe 1, Jeffrey Thompson 1, Xiaozhe Han 1, Toshihisa Kawai 1, Maiko Suzuki 1
1Department of Oral Science and Translational Research, College of Dental Medicine, Nova Southeastern University
Microhardness is a mechanical property and an informative parameter for evaluating hard tissue pathophysiology. Here, we demonstrate a standardized protocol (sample preparation, polishing, flat surface, and indentation sites) for microhardness analysis in tooth and alveolar bone in rodent oral disease models, namely, dental fluorosis, and ligature-induced periodontal bone resorption.
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