Accedi

Nova Southeastern University

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors

Andrea Linares¹, Leena E. Couling², Eduardo J. Carrera³, Robert C. Speth²

¹Farquhar College of Arts and Sciences, Nova Southeastern University, ²Department of Pharmaceutical Sciences, College of Pharmacy, Nova Southeastern University, ³School of Medicine, University of Colorado

Here we present a protocol to describe the localization of angiotensin II Type 1 receptors in the rat brain by quantitative, densitometric, in vitro receptor autoradiography using an iodine-125 labeled analog of angiotensin II.

Biology

Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models

Motoki Okamoto^*1, Shohei Yamashita^*1, Takumi Memida¹, Melanie Mendonca¹, Susanne Brueckner¹, Shin Nakamura¹, Satoru Shindo¹, Ria Achong-Bowe¹, Jeffrey Thompson¹, Xiaozhe Han¹, Toshihisa Kawai¹, Maiko Suzuki¹

¹Department of Oral Science and Translational Research, College of Dental Medicine, Nova Southeastern University

Microhardness is a mechanical property and an informative parameter for evaluating hard tissue pathophysiology. Here, we demonstrate a standardized protocol (sample preparation, polishing, flat surface, and indentation sites) for microhardness analysis in tooth and alveolar bone in rodent oral disease models, namely, dental fluorosis, and ligature-induced periodontal bone resorption.