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Hasselt University

2 ARTICLES PUBLISHED IN JoVE

Medicine

Fundus Photography as a Convenient Tool to Study Microvascular Responses to Cardiovascular Disease Risk Factors in Epidemiological Studies

Patrick De Boever^1,2, Tijs Louwies^1,2, Eline Provost^1,2, Luc Int Panis^1,3, Tim S. Nawrot^2,4

¹Environmental Risk and Health, Flemish Institute for Technological Research (VITO), ²Centre for Environmental Sciences, Hasselt University, ³Transportation Research Institute, Hasselt University, ⁴Department of Public Health, Occupational and Environmental Medicine, Leuven University

Retinal image analysis is an unobtrusive procedure for visualizing the microcirculation. The impact of cardiovascular disease risk factors can result in changes of retinal vessel calibers. The procedures to acquire fundus images and the steps for calculating the vessel calibers are described.

Medicine

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

Suzan Wetzels^*1,2,3, Mitchell Bijnen^*1,2, Erwin Wijnands^2,4, Erik A.L. Biessen^2,4, Casper G. Schalkwijk^1,2, Kristiaan Wouters^1,2

¹Department of Internal Medicine, MUMC, ²CARIM, MUMC, ³Department of Immunology and Biochemistry, Biomedical Research Institute, Hasselt University, ⁴Department of Pathology, MUMC

This article describes a method to analyze immune cell content of adipose tissue by isolation of immune cells from adipose tissue and subsequent analysis using flow cytometry.