Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM ProtocolAnne C. Wolfes 1,2,3, Camin Dean 3
1Chemical Biology, Chemistry Research Laboratory, University of Oxford, 2Department of Physiology, Anatomy, and Genetics, University of Oxford, 3Trans-synaptic signaling, European Neuroscience Institute
The AWESAM protocol described here is optimal for culturing murine astrocytes in isolation from other brain cells in a fast, simple, and inexpensive manner. AWESAM astrocytes exhibit spontaneous Ca2+ signaling, morphology, and gene expression profiles similar to astrocytes in vivo.
